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eIF4B (Phospho-Ser422) Colorimetric Cell-Based ELISA Kit

SKU:
CBCAB01340
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Metabolism
Reactivity:
Human
Mouse
Detection Method:
Colorimetric
$719
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Description

system_update_altDatasheet
Product Name:eIF4B (Phospho-Ser422) Colorimetric Cell-Based ELISA
Product Code:CBCAB01340
ELISA Type:Cell-Based
Target:eIF4B (Phospho-Ser422)
Reactivity:Human, Mouse
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Format:2 x 96-Well Microplates

The eIF4B (Phospho-Ser422) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect eIF4B protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated eIF4B in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on eIF4B phosphorylation.

Qualitative determination of eIF4B (Phospho-Ser422) concentration is achieved by an indirect ELISA format. In essence, eIF4B (Phospho-Ser422) is captured by eIF4B (Phospho-Ser422)-specific primary (1ø) antibodies while the HRP-conjugated secondary (2ø) antibodies bind the Fc region of the 1ø antibody. Through this binding, the HRP enzyme conjugated to the 2ø antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 3716, UniProt ID: P23588, OMIM: 147795, Unigene: Hs.207538
Gene Symbol:EIF4B
Sub Type:Phospho
UniProt Protein Function:EIF4B: eukaryotic translation initiation factor 4B. Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
UniProt Protein Details:

Protein type:RNA-binding; Translation initiation; Translation

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytosol; eukaryotic translation initiation factor 4F complex; polysome

Molecular Function:helicase activity; protein binding; ribosomal small subunit binding; RNA binding; RNA strand annealing activity; RNA strand-exchange activity

Biological Process: formation of translation preinitiation complex; poly(A) tail shortening; regulation of translational initiation; translational initiation

UniProt Code:P23588
NCBI GenInfo Identifier:205371761
NCBI Gene ID:1975
NCBI Accession:P23588.2
UniProt Secondary Accession:P23588,Q4G0E3, Q53HQ2, Q6GPH5, Q6IB46, Q8WYK5, B4DS13
UniProt Related Accession:P23588
Molecular Weight:64,805 Da
NCBI Full Name:Eukaryotic translation initiation factor 4B
NCBI Synonym Full Names:eukaryotic translation initiation factor 4B
NCBI Official Symbol:EIF4B  
NCBI Official Synonym Symbols:EIF-4B; PRO1843  
NCBI Protein Information:eukaryotic translation initiation factor 4B
UniProt Protein Name:Eukaryotic translation initiation factor 4B
Protein Family:Eukaryotic translation initiation factor
UniProt Gene Name:EIF4B  
UniProt Entry Name:IF4B_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37 °C prior to adding cells.
2.Incubate the cells for overnight at 37 °C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4 °C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies Anti-eIF4B (Phospho-Ser422) Antibody, Anti-eIF4B Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4 °C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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