Anti-PI3 Antibody (CAB5441)

Antibody Name:	Anti-PI3 Antibody
Antibody SKU:	CAB5441
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 23-117 of human PI3 (NP_002629.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	U-87MG, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 23-117 of human PI3 (NP_002629.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AVTG VPVK GQDT VKGR VPFN GQDP VKGQ VSVK GQDK VKAQ EPVK GPVS TKPG SCPI ILIR CAML NPPN RCLK DTDC PGIK KCCE GSCG MACF VPQ
Gene ID:	5266
Uniprot:	P19957
Cellular Location:	Secreted
Calculated MW:	12kDa
Observed MW:	12kDa

Synonyms:

PI3, ESI, SKALP, WAP3, WFDC14, cementoin, elafin

Background:

This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.

UniProt Protein Function:	elafin: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
UniProt Protein Details:	Protein type:Extracellular matrix; Inhibitor; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: proteinaceous extracellular matrix Molecular Function:serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity Biological Process: copulation
NCBI Summary:	This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq, Oct 2008]
UniProt Code:	P19957
NCBI GenInfo Identifier:	119262
NCBI Gene ID:	5266
NCBI Accession:	P19957.3
UniProt Secondary Accession:	P19957,Q6FG74, E1P618,
UniProt Related Accession:	P19957
Molecular Weight:	12,270 Da
NCBI Full Name:	Elafin
NCBI Synonym Full Names:	peptidase inhibitor 3, skin-derived
NCBI Official Symbol:	PI3
NCBI Official Synonym Symbols:	ESI; WAP3; SKALP; WFDC14; cementoin
NCBI Protein Information:	elafin; PI-3; trappin-2; pre-elafin; protease inhibitor WAP3; elastase-specific inhibitor; skin-derived antileukoproteinase; WAP four-disulfide core domain 14; WAP four-disulfide core domain protein 14; protease inhibitor 3, skin-derived (SKALP)
UniProt Protein Name:	Elafin
UniProt Synonym Protein Names:	Elastase-specific inhibitor; ESI; Peptidase inhibitor 3; PI-3; Protease inhibitor WAP3; Skin-derived antileukoproteinase; SKALP; WAP four-disulfide core domain protein 14
Protein Family:	Elafin
UniProt Gene Name:	PI3
UniProt Entry Name:	ELAF_HUMAN

	Western blot analysis of extracts of various cell lines, using PI3 antibody (CAB5441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human colon using PI3 antibody (CAB5441) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using PI3 antibody (CAB5441) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-PI3 Antibody (CAB12482)	Human Elafin (PI3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)