Anti-TCEB1 Antibody (CAB1989)

Antibody Name:	Anti-TCEB1 Antibody
Antibody SKU:	CAB1989
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-112 of human TCEB1 (NP_001191791.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, HepG2, Mouse testis, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-112 of human TCEB1 (NP_001191791.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDGE EKTY GGCE GPDA MYVK LISS DGHE FIVK REHA LTSG TIKA MLSG PGQF AENE TNEV NFRE IPSH VLSK VCMY FTYK VRYT NSST EIPE FPIA PEIA LELL MAAN FLDC
Gene ID:	6921
Uniprot:	Q15369
Cellular Location:	Nucleus
Calculated MW:	10kDa/12kDa
Observed MW:	12kDa

Synonyms:

ELOC, SIII, TCEB1, eloC, elongin-C

Background:

This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been identified.

UniProt Protein Function:	TCEB1: SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). Belongs to the SKP1 family.
UniProt Protein Details:	Protein type:Transcription initiation complex Chromosomal Location of Human Ortholog: 8q21.11 Cellular Component: cytosol; nucleoplasm Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: positive regulation of RNA elongation from RNA polymerase II promoter; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of transcription from RNA polymerase II promoter; RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been identified. [provided by RefSeq, Mar 2011]
UniProt Code:	Q15369
NCBI GenInfo Identifier:	32699511
NCBI Gene ID:	6921
NCBI Accession:	Q15369.1
UniProt Secondary Accession:	Q15369,Q567Q6, E5RGD9,
UniProt Related Accession:	Q15369
Molecular Weight:	10,832 Da
NCBI Full Name:	Transcription elongation factor B polypeptide 1
NCBI Synonym Full Names:	transcription elongation factor B subunit 1
NCBI Official Symbol:	TCEB1
NCBI Official Synonym Symbols:	SIII; eloC
NCBI Protein Information:	transcription elongation factor B polypeptide 1
UniProt Protein Name:	Transcription elongation factor B polypeptide 1
UniProt Synonym Protein Names:	Elongin 15 kDa subunit; Elongin-C; EloC; RNA polymerase II transcription factor SIII subunit C; SIII p15
Protein Family:	Transcription elongation factor B polypeptide
UniProt Gene Name:	TCEB1
UniProt Entry Name:	ELOC_HUMAN

	Western blot analysis of extracts of various cell lines, using TCEB1 antibody (CAB1989) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse liver using TCEB1 antibody (CAB1989) (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using TCEB1 antibody (CAB1989) (40x lens).

Antibodies

Anti-TCEB1 Antibody (CAB12515)

TCEB1 Antibody (PACO12668)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)