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Anti-ELSPBP1 Antibody (CAB2151)

SKU:
CAB2151
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology
$167 - $503
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Description

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Antibody Name:Anti-ELSPBP1 Antibody
Antibody SKU:CAB2151
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human ELSPBP1 (NP_071425.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HepG2, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human ELSPBP1 (NP_071425.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTRW SSYL LGWT TFLL YSYE SSGG MHEE CVFP FTYK GSVY FTCT HIHS LSPW CATR AVYN GQWK YCQS EDYP RCIF PFIY RGKA YNSC ISQG SFLG SLWC SVTS VFDE KQQW KFCE TNEY GGNS LRKP CIFP SIYR NNVV SDCM EDES NKLW CPTT ENMD KDGK WSFC ADTR ISAL VPGF PCHF PFNY KNKN YFNC TNEG SKEN LVWC ATSY NYDQ DHTW VYC
Gene ID:64100
Uniprot:Q96BH3
Cellular Location:Secreted
Calculated MW:26kDa
Observed MW:34kDa
Synonyms:ELSPBP1, E12, EDDM12, EL149, HE12
Background:The protein encoded by this gene belongs to the sperm-coating protein family of epididymal origin. This protein and its canine homolog are the first known examples of proteins with four tandemly arranged fibronectin type 2 (Fn2) domains in the Fn2-module protein family.
UniProt Protein Function:ELSPBP1: Binds to spermatozoa upon ejaculation and may play a role in sperm capacitation. Has phosphorylcholine-binding activity. Belongs to the seminal plasma protein family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular region

Biological Process: single fertilization

NCBI Summary:The protein encoded by this gene belongs to the sperm-coating protein family of epididymal origin. This protein and its canine homolog are the first known examples of proteins with four tandemly arranged fibronectin type 2 (Fn2) domains in the Fn2-module protein family. [provided by RefSeq, Jul 2008]
UniProt Code:Q96BH3
NCBI GenInfo Identifier:296434494
NCBI Gene ID:64100
NCBI Accession:Q96BH3.2
UniProt Related Accession:Q96BH3
Molecular Weight:34kDa
NCBI Full Name:Epididymal sperm-binding protein 1
NCBI Synonym Full Names:epididymal sperm binding protein 1
NCBI Official Symbol:ELSPBP1  
NCBI Official Synonym Symbols:E12; HE12; EL149; EDDM12  
NCBI Protein Information:epididymal sperm-binding protein 1
UniProt Protein Name:Epididymal sperm-binding protein 1
UniProt Synonym Protein Names:Epididymal secretory protein 12; hE12
Protein Family:Epididymal sperm-binding protein
UniProt Gene Name:ELSPBP1  
UniProt Entry Name:ESPB1_HUMAN
Anti-ELSPBP1 Antibody (CAB2151)Western blot analysis of extracts of various cell lines, using ELSPBP1 antibody (CAB2151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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