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Anti-Endo G Antibody (CAB0604)

SKU:
CAB0604
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 1 Anti-Endo G Antibody CAB0604
  • Cell Biology Antibodies 1 Anti-Endo G Antibody CAB0604
  • Cell Biology Antibodies 1 Anti-Endo G Antibody CAB0604
€139 - €419
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Description

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Antibody Name:Anti-Endo G Antibody
Antibody SKU:CAB0604
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 49-297 of human Endo G (NP_004426.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, MCF7, SW620, Mouse heart, Mouse kidney, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 49-297 of human Endo G (NP_004426.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AELP PVPG GPRG PGEL AKYG LPGL AQLK SRES YVLC YDPR TRGA LWVV EQLR PERL RGDG DRRE CDFR EDDS VHAY HRAT NADY RGSG FDRG HLAA AANH RWSQ KAMD DTFY LSNV APQV PHLN QNAW NNLE KYSR SLTR SYQN VYVC TGPL FLPR TEAD GKSY VKYQ VIGK NHVA VPTH FFKV LILE AAGG QIEL RTYV MPNA PVDE AIPL ERFL VPIE SIER ASGL LFVP NILA RAGS LKAI TAGS K
Gene ID:2021
Uniprot:Q14249
Cellular Location:Mitochondrion
Calculated MW:32kDa
Observed MW:33kDa
Synonyms:ENDOG
Background:The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
UniProt Protein Function:ENDOG: Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. Homodimer. Belongs to the DNA/RNA non-specific endonuclease family.
UniProt Protein Details:

Protein type:EC 3.1.30.-; Ribonuclease; Deoxyribonuclease; Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: mitochondrion

Molecular Function:protein binding; nuclease activity; nucleic acid binding; metal ion binding; endonuclease activity

Biological Process: response to antibiotic; positive regulation of apoptosis; in utero embryonic development; DNA fragmentation during apoptosis; DNA recombination

NCBI Summary:The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. [provided by RefSeq, Jul 2008]
UniProt Code:Q14249
NCBI GenInfo Identifier:24638471
NCBI Gene ID:2021
NCBI Accession:Q14249.3
UniProt Secondary Accession:Q14249,Q5T281, Q9BSP2,
UniProt Related Accession:Q14249
Molecular Weight:32,620 Da
NCBI Full Name:Endonuclease G, mitochondrial
NCBI Synonym Full Names:endonuclease G
NCBI Official Symbol:ENDOG  
NCBI Protein Information:endonuclease G, mitochondrial; endo G; mitochondrial endonuclease G
UniProt Protein Name:Endonuclease G, mitochondrial
Protein Family:Endoglin
UniProt Gene Name:ENDOG  
UniProt Entry Name:NUCG_HUMAN
Anti-Endo G Antibody (CAB0604)Western blot analysis of extracts of various cell lines, using Endo G antibody (CAB0604) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-Endo G Antibody (CAB0604)Immunohistochemistry of paraffin-embedded mouse kidney using Endo G antibody (CAB0604) at dilution of 1:100 (40x lens).
Anti-Endo G Antibody (CAB0604)Immunohistochemistry of paraffin-embedded mouse lung using Endo G antibody (CAB0604) at dilution of 1:100 (40x lens).

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