The Equine Interleukin-1 Alpha (IL1A) ELISA Kit from Assay Genie is specifically designed for the accurate detection of interleukin-1 alpha levels in equine serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring trustworthy and reproducible results that are suitable for a wide range of research applications.Interleukin-1 alpha is a key cytokine involved in the immune response and inflammation regulation. Its dysregulation has been linked to various inflammatory diseases and conditions in horses, such as arthritis, respiratory issues, and infectious diseases.
Studying interleukin-1 alpha levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of targeted therapeutic interventions.Order the Equine Interleukin-1 Alpha (IL1A) ELISA Kit from Assay Genie today and unlock the potential for groundbreaking research and discoveries in equine health and disease.
Product Name:
Equine Interleukin 1 Alpha (IL-1a) ELISA Kit
Product Code:
HRDL00015
Size:
96 Assays
Target:
IL1a
Synonyms:
IL1-A, IL-1?, IL1F1, Preinterleukin 1 Alpha, Hematopoietin-1, Pro-Interleukin-1-Alpha, Interleukin-1 Family Member 1
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
