Anti-ERCC3 Antibody (CAB1714)

Antibody Name:	Anti-ERCC3 Antibody
Antibody SKU:	CAB1714
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 513-782 of human ERCC3 (NP_000113.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, Mouse testis, Mouse thymus, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 513-782 of human ERCC3 (NP_000113.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PMSP EFYR EYVA IKTK KRIL LYTM NPNK FRAC QFLI KFHE RRND KIIV FADN VFAL KEYA IRLN KPYI YGPT SQGE RMQI LQNF KHNP KINT IFIS KVGD TSFD LPEA NVLI QISS HGGS RRQE AQRL GRVL RAKK GMVA EEYN AFFY SLVS QDTQ EMAY STKR QRFL VDQG YSFK VITK LAGM EEED LAFS TKEE QQQL LQKV LAAT DLDA EEEV VAGE FGSR SSQA SRRF GTMS SMSG ADDT VYME YHSS RSKA PSKH VHPL FKRF RK
Gene ID:	2071
Uniprot:	P19447
Cellular Location:	Nucleus
Calculated MW:	89kDa
Observed MW:	89kDa

Synonyms:

ERCC3, BTF2, GTF2H, RAD25, TFIIH, TTD2, XPB

Background:

This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	XPB: ATP-dependent 3'-5' DNA helicase, component of the core- TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage. One of the 6 subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. Interacts with PUF60. Interacts with ATF7IP. Interacts with Epstein-Barr virus EBNA2. Belongs to the helicase family. RAD25/XPB subfamily.
UniProt Protein Details:	Protein type:Helicase; DNA repair, damage; EC 3.6.4.12; Transcription factor Chromosomal Location of Human Ortholog: 2q21 Cellular Component: nucleoplasm; holo TFIIH complex; nucleus Molecular Function:protein C-terminus binding; DNA-dependent ATPase activity; GTP binding; ATPase activity; dATP binding; 3'-5' DNA helicase activity; protein N-terminus binding; peptide binding; transcription factor binding; protein kinase activity; ATP-dependent DNA helicase activity; RNA polymerase subunit kinase activity; protein binding; DNA binding; damaged DNA binding; ATP binding Biological Process: transcription from RNA polymerase II promoter; DNA topological change; viral reproduction; apoptosis; positive regulation of apoptosis; positive regulation of viral transcription; protein amino acid phosphorylation; regulation of gene expression, epigenetic; protein localization; mRNA capping; UV protection; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; response to UV; transcription initiation from RNA polymerase II promoter; hair cell differentiation; nucleotide-excision repair, DNA incision; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; termination of RNA polymerase I transcription; DNA repair; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; response to hypoxia; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; transcription initiation from RNA polymerase I promoter Disease: Trichothiodystrophy 2, Photosensitive; Xeroderma Pigmentosum, Complementation Group B
NCBI Summary:	This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Code:	P19447
NCBI GenInfo Identifier:	119541
NCBI Gene ID:	2071
NCBI Accession:	P19447.1
UniProt Related Accession:	P19447
Molecular Weight:	80kDa
NCBI Full Name:	General transcription and DNA repair factor IIH helicase subunit XPB
NCBI Synonym Full Names:	ERCC excision repair 3, TFIIH core complex helicase subunit
NCBI Official Symbol:	ERCC3
NCBI Official Synonym Symbols:	XPB; BTF2; TTD2; GTF2H; RAD25; TFIIH
NCBI Protein Information:	general transcription and DNA repair factor IIH helicase subunit XPB
UniProt Protein Name:	TFIIH basal transcription factor complex helicase XPB subunit
UniProt Synonym Protein Names:	Basic transcription factor 2 89 kDa subunit; BTF2 p89; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH 89 kDa subunit; TFIIH p89; Xeroderma pigmentosum group B-complementing protein
Protein Family:	Probable DNA repair helicase
UniProt Gene Name:	ERCC3
UniProt Entry Name:	ERCC3_HUMAN

	Western blot analysis of extracts of various cell lines, using ERCC3 antibody (CAB1714) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded mouse lung using ERCC3 antibody (CAB1714) at dilution of 1:200 (40x lens).

Antibodies

Anti-ERCC3 Antibody (CAB12702)

ERCC3 Antibody (PACO09129)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)