Description
EV71 P3C/Protease 3C Recombinant Protein (N-His)
EV71 P3C/Protease 3C Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gly1-Gln183, with an N-terminal His tag. Predicted molecular weight: 22.45 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Genome polyprotein (P3C/Protease 3C) is a ~242 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. For research use only.
The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1, together with VP2, interacts with host cell receptor SCARB2 to provide virion attachment to target host cells. This attachment induces virion internalization.
| Product Name: | EV71 P3C/Protease 3C Recombinant Protein (N-His) |
| SKU: | GXRP08213 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | EV71 |
| Synonyms: | 3C protease, partial, 3C protease, partial |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gly1-Gln183 |
| Calculated MW: | 22.45 kDa |
| UniProt: | B9VUU3 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
