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Western blot analysis of various lysates, using F4/80 Rabbit mAb (CAB23788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

F4/80 Monoclonal Antibody (CAB23788)

Datasheet MSDS
SKU CAB23788
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Mouse, Rat
Host Species Rabbit
Synonyms Emr1, Ly71, F4/80, Gpf480, TM7LN3, DD7A5-7, EGF-TM7
$173 - $603

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Description

system_update_altDatasheetsystem_update_altMSDS

F4/80 Monoclonal Antibody (CAB23788)

The F4/80 Monoclonal Antibody (CAB23788) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, specifically targets the F4/80 protein found on the surface of macrophages in mouse samples. With a high level of specificity and sensitivity, the F4/80 Monoclonal Antibody is suitable for use in various applications including immunohistochemistry, flow cytometry, and immunofluorescence. Its ability to bind to the F4/80 protein allows for precise detection and analysis of macrophages in different tissues and biological samples.

This antibody is validated for use in IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.

Product Name:F4/80 Monoclonal Antibody
SKU:CAB23788
Size:20μL, 100μL
Reactivity:Mouse, Rat
Clone Number:ARC61555
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:KRIL FKCK EDLI LQSE QIQQ CQAV QGRD LGYA SFCT LVNA TFTI LDNT CENK SAPV SLQS AATS VSLV LEQA TTWF ELSK EETS TLGT ILLE TVES TMLA ALLI PSGN ASQM IQTE YLDI ESKV INEE CKEN ESIN LAAR GDKM NVGC FIIK ESVS TGAP GVAF VSFA HMES VLNE RFFE DGQS FRKL RMNS RVVG GTVT GEKK EDFS KPII YTLQ HIQP KQKS ERPI CVSW NTDV EDGR WTPS GCEI VEAS ETHT VCSC NRMA NLAI IMAS GELT MEF
Tested Applications:IHC-P ELISA IF-P
Recommended Dilution:
IF-P1:100 - 1:400
IHC-P1:300 - 1:1200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:Emr1, Ly71, F4/80, Gpf480, TM7LN3, DD7A5-7, EGF-TM7
Cellular Localization:Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:102kDa
Observed MW:Refertofigures

Predicted to enable G protein-coupled receptor activity. Predicted to be involved in adenylate cyclase-activating G protein-coupled receptor signaling pathway. Predicted to act upstream of or within G protein-coupled receptor signaling pathway and adaptive immune response. Located in external side of plasma membrane. Is expressed in several structures, including cardiovascular system; central nervous system; genitourinary system; hemolymphoid system; and intestine. Orthologous to human ADGRE1 (adhesion G protein-coupled receptor E1).

Purification MethodAffinity purification
Gene ID13733
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse liver tissue using F4/80 Rabbit mAb (CAB23788) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain tissue using F4/80 Rabbit mAb (CAB23788) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse testis tissue using F4/80 Rabbit mAb (CAB23788) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse thymus tissue using F4/80 Rabbit mAb (CAB23788) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat liver tissue using F4/80 Rabbit mAb (CAB23788) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageConfocal imaging of paraffin-embedded Mouse spleen tissue using F4/80 Rabbit mAb (CAB23788, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Product ImageConfocal imaging of paraffin-embedded Mouse liver tissue using F4/80 Rabbit mAb (CAB23788, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
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