Description
FANCD2 Antibody (CAB2072)
The FANCD2 Antibody (CAB2072) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding to the FANCD2 protein, this antibody enables the detection and analysis of FANCD2 in various cell types, making it a crucial tool for studies in molecular biology and cancer research.FANCD2 plays a critical role in maintaining genomic stability and protecting cells from DNA damage. Mutations in the FANCD2 gene are associated with Fanconi anemia, a rare genetic disorder characterized by bone marrow failure and an increased risk of cancer.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Rat samples.
| Product Name: | FANCD2 Antibody |
| SKU: | CAB2072 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||||
| Sequence: | AFSK QNEA SSHI QDDM HLVI RKQL SSTV FKYK LIGI IGAV TMAG IMAA DRSE SPSL TQER ANLS DEQC TQVT SLLQ LVHS CSEQ SPQA SALY YDEF ANLI QHEK LDPK ALEW VGHT ICND FQDA FVVD SCVV PEGD FPFP VKAL YGLE EYDT QDGI AINL LPLL FSQD FAKD GGPV TSQE S | ||||||||
| Tested Applications: | WB IF/ICC IP ELISA | ||||||||
| Recommended Dilution: |
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| Synonyms: | FA4, FAD, FACD, FAD2, FA-D2, FANCD, FANCD2 |
| Positive Sample: | MCF7, HeLa, K-562, Jurkat, A-549, K-562 |
| Cellular Localization: | Nucleus. |
| Calculated MW: | 164kDa |
| Observed MW: | 180kDa/164kDa |
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in multiple transcript variants.
| Purification Method | Affinity purification |
| Gene ID | 2177 |
| RRID | AB_2764094 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from K-562 cells using FANCD2 Rabbit pAb (CAB2072) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using FANCD2 Rabbit pAb (CAB2072) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200 μg extracts of A-549 cells using 3 μg FANCD2 antibody (CAB2072). Western blot was performed from the immunoprecipitate using FANCD2 antibody (CAB2072) at a dilution of 1:1000. |
