Anti-FAP Antibody (CAB6349)

Antibody Name:	Anti-FAP Antibody
Antibody SKU:	CAB6349
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-280 of human FAP (NP_004451.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-280 of human FAP (NP_004451.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LRPS RVHN SEEN TMRA LTLK DILN GTFS YKTF FPNW ISGQ EYLH QSAD NNIV LYNI ETGQ SYTI LSNR TMKS VNAS NYGL SPDR QFVY LESD YSKL WRYS YTAT YYIY DLSN GEFV RGNE LPRP IQYL CWSP VGSK LAYV YQNN IYLK QRPG DPPF QITF NGRE NKIF NGIP DWVY EEEM LATK YALW WSPN GKFL AYAE FNDT DIPV IAYS YYGD EQYP RTIN IPYP KAGA KNPV VRIF IIDT TYPA YVGP QEV
Gene ID:	2191
Uniprot:	Q12884
Cellular Location:	Cell membrane, Cell projection, Cell surface, Cytoplasm, Membrane, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein, invadopodium membrane, lamellipodium membrane, ruffle membrane
Calculated MW:	26kDa/87kDa
Observed MW:	88kDa

Synonyms:

FAP, DPPIV, FAPA, FAPalpha, SIMP

Background:

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	FAP: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. Belongs to the peptidase S9B family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.14.5; Protease; EC 3.4.21.26; Membrane protein, integral Chromosomal Location of Human Ortholog: 2q23 Cellular Component: apical part of cell; cell surface; cytoplasm; extracellular space; focal adhesion; integral to membrane; lamellipodium; plasma membrane Molecular Function:dipeptidyl-peptidase activity; endopeptidase activity; integrin binding; metalloendopeptidase activity; peptidase activity; protease binding; protein binding; protein dimerization activity; protein homodimerization activity; serine-type endopeptidase activity; serine-type peptidase activity Biological Process: angiogenesis; cell adhesion; endothelial cell migration; proteolysis; proteolysis involved in cellular protein catabolic process; regulation of fibrinolysis
NCBI Summary:	The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
UniProt Code:	Q12884
NCBI GenInfo Identifier:	292495099
NCBI Gene ID:	2191
NCBI Accession:	Q12884.5
UniProt Secondary Accession:	Q12884,O00199, Q53TP5, Q86Z29, Q99998, Q9UID4,
UniProt Related Accession:	Q12884
Molecular Weight:
NCBI Full Name:	Prolyl endopeptidase FAP
NCBI Synonym Full Names:	fibroblast activation protein alpha
NCBI Official Symbol:	FAP
NCBI Official Synonym Symbols:	FAPA; SIMP; DPPIV
NCBI Protein Information:	prolyl endopeptidase FAP
UniProt Protein Name:	Prolyl endopeptidase FAP
UniProt Synonym Protein Names:	170 kDa melanoma membrane-bound gelatinase
Protein Family:	Prolyl endopeptidase
UniProt Gene Name:	FAP
UniProt Entry Name:	SEPR_HUMAN

	Western blot analysis of extracts of mouse heart, using FAP antibody (CAB6349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using FAP antibody (CAB6349) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-FAP1 Antibody (CAB11572)	FAP-1 Colorimetric Cell-Based ELISA Kit
FAP Antibody (PACO06958)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)