Anti-FAS Antibody (CAB12401)

Antibody Name:	Anti-FAS Antibody
Antibody SKU:	CAB12401
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-335 of human FAS (NP_000034.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human
Positive Samples:	HT-1080, HepG2, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-335 of human FAS (NP_000034.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	INSK GLEL RKTV TTVE TQNL EGLH HDGQ FCHK PCPP GERK ARDC TVNG DEPD CVPC QEGK EYTD KAHF SSKC RRCR LCDE GHGL EVEI NCTR TQNT KCRC KPNF FCNS TVCE HCDP CTKC EHGI IKEC TLTS NTKC KEEG SRSN LGWL CLLL LPIP LIVW VKRK EVQK TCRK HRKE NQGS HESP TLNP ETVA INLS DVDL SKYI TTIA GVMT LSQV KGFV RKNG VNEA KIDE IKND NVQD TAEQ KVQL LRNW HQLH GKKE AYDT LIKD LKKA NLCT LAEK IQTI ILKD ITSD SENS NFRN EIQS LV
Gene ID:	355
Uniprot:	P25445
Cellular Location:	Cell membrane, Secreted, Single-pass type I membrane protein
Calculated MW:	9kDa/11kDa/14kDa/16kDa/24kDa/35kDa/37kDa
Observed MW:	40-50KDa

Synonyms:

ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6, FAS

Background:

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.

UniProt Protein Function:	FAS: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Apoptosis; Membrane protein, integral; Receptor, cytokine; Cell surface Chromosomal Location of Human Ortholog: 10q24.1 Cellular Component: cell surface; cytoplasm; plasma membrane; integral to membrane; CD95 death-inducing signaling complex; nucleus; cytosol; lipid raft; external side of plasma membrane Molecular Function:identical protein binding; protein binding; signal transducer activity; transmembrane receptor activity; receptor activity; kinase binding Biological Process: circadian rhythm; caspase activation; spleen development; regulation of myeloid cell differentiation; renal system process; transformed cell apoptosis; apoptosis; positive regulation of apoptosis; positive regulation of protein homooligomerization; regulation of lymphocyte differentiation; response to toxin; response to glucocorticoid stimulus; negative regulation of B cell activation; signal transduction; negative thymic T cell selection; regulation of apoptosis; inflammatory cell apoptosis; B cell mediated immunity; induction of apoptosis via death domain receptors; gene expression; protein complex assembly; immunoglobulin production; activated T cell apoptosis; protein homooligomerization; negative regulation of apoptosis Disease: Autoimmune Lymphoproliferative Syndrome
NCBI Summary:	The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]
UniProt Code:	P25445
NCBI GenInfo Identifier:	119833
NCBI Gene ID:	355
NCBI Accession:	P25445.1
UniProt Secondary Accession:	P25445,Q14292, Q14293, Q14294, Q14295, Q16652, Q5T9P1 Q5T9P2, Q5T9P3, Q6SSE9, A9UJX4, B6VNV4,
UniProt Related Accession:	P25445
Molecular Weight:	37,732 Da
NCBI Full Name:	Tumor necrosis factor receptor superfamily member 6
NCBI Synonym Full Names:	Fas cell surface death receptor
NCBI Official Symbol:	FAS
NCBI Official Synonym Symbols:	APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
NCBI Protein Information:	tumor necrosis factor receptor superfamily member 6; Fas AMA; FAS 827dupA; CD95 antigen; FASLG receptor; apoptosis antigen 1; Delta Fas/APO-1/CD95; FAS receptor variant 9; APO-1 cell surface antigen; TNF receptor superfamily member 6; apoptosis-mediating surface antigen FAS; Fas (TNF receptor superfamily, member 6); tumor necrosis factor receptor superfamily, member 6
UniProt Protein Name:	Tumor necrosis factor receptor superfamily member 6
UniProt Synonym Protein Names:	Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor
Protein Family:	Development-specific protein
UniProt Gene Name:	FAS
UniProt Entry Name:	TNR6_HUMAN

	Western blot analysis of extracts of various cell lines, using FAS antibody (CAB12401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using FAS antibody (CAB12401). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-FAS Antibody (CAB0233)	Human FAS/CD95 (Factor Related Apoptosis) CLIA Kit (HUES00065)
Anti-FAS Antibody (CAB2639)	Human FAS/CD95 (Factor Related Apoptosis) ELISA Kit (HUES01354)
Anti-Phospho-FAS-Y291 Antibody (CABP0007)	Human Tumor necrosis factor receptor superfamily member 6 (FAS) ELISA Kit
Anti-Fas Antibody [KO Validated] (CAB19582)	FAS Colorimetric Cell-Based ELISA Kit
FAS Antibody (PACO00791)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)