Western blot analysis of various lysates, using FceR1 alpha antibody (CAB22729) at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The FceR1 alpha Monoclonal Antibody (CAB22729) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced using advanced technology, exhibits high specificity and sensitivity towards FCER1 Alpha in human samples, making it an ideal choice for various research applications including Western blotting.FCER1 Alpha, also known as Fc epsilon RI alpha chain, plays a crucial role in the activation of mast cells and basophils, key players in allergic reactions and inflammatory responses. By targeting FCER1 Alpha, researchers can gain valuable insights into the mechanisms underlying allergic diseases, asthma, and other immune-mediated disorders.
This antibody is validated for use in - applications and has demonstrated reactivity against - samples.
Product Name:
FceR1 alpha Monoclonal Antibody
SKU:
CAB22729
Size:
20μL, 100μL
Tested Applications:
-
Recommended Dilution:
Western blot analysis of various lysates, using FceR1 alpha Rabbit mAb (CAB22729) at 1:6000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Western blot analysis of various lysates using FceR1 alpha Rabbit mAb (CAB22729) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): MOLT-4 Exposure time: 60s.
