Anti-FHIT Antibody (CAB1196)

Antibody Name:	Anti-FHIT Antibody
Antibody SKU:	CAB1196
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-147 of human FHIT (NP_001159715.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HeLa, Jurkat, 293T, Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-147 of human FHIT (NP_001159715.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LVNR KPVV PGHV LVCP LRPV ERFH DLRP DEVA DLFQ TTQR VGTV VEKH FHGT SLTF SMQD GPEA GQTV KHVH VHVL PRKA GDFH RNDS IYEE LQKH DKED FPAS WRSE EEMA AEAA ALRV YFQ
Gene ID:	2272
Uniprot:	P49789
Cellular Location:	Cytoplasm, Mitochondrion, Nucleus
Calculated MW:	16kDa
Observed MW:	17kDa

Synonyms:

FHIT, AP3Aase, FRA3B

Background:

This gene, a member of the histidine triad gene family, encodes a diadenosine 5', 5'''-P1, P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	FHIT: a member of the histidine triad gene family. A diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. Its gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. A possible tumor suppressor in specific tissues. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines. Aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas.
UniProt Protein Details:	Protein type:EC 3.6.1.29; Hydrolase; Motility/polarity/chemotaxis; DNA replication; Nucleotide Metabolism - purine; Tumor suppressor Chromosomal Location of Human Ortholog: 3p14.2 Cellular Component: cytoplasm; cytosol Molecular Function:bis(5'-adenosyl)-triphosphatase activity; catalytic activity; hydrolase activity; identical protein binding; protein binding; ubiquitin protein ligase binding Biological Process: negative regulation of proteasomal ubiquitin-dependent protein catabolic process; nucleotide metabolic process; purine nucleotide metabolic process
NCBI Summary:	This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:	P49789
NCBI GenInfo Identifier:	1706794
NCBI Gene ID:	2272
NCBI Accession:	P49789.3
UniProt Secondary Accession:	P49789,Q45QG9, Q6IU12, A2IAS9, A2IAT0, A2IAT6, A8K1A9
UniProt Related Accession:	P49789
Molecular Weight:	16,858 Da
NCBI Full Name:	Bis(5'-adenosyl)-triphosphatase
NCBI Synonym Full Names:	fragile histidine triad
NCBI Official Symbol:	FHIT
NCBI Official Synonym Symbols:	FRA3B; AP3Aase
NCBI Protein Information:	bis(5'-adenosyl)-triphosphatase
UniProt Protein Name:	Bis(5'-adenosyl)-triphosphatase
UniProt Synonym Protein Names:	AP3A hydrolase; AP3Aase; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein
Protein Family:	Bis(5'-adenosyl)-triphosphatase
UniProt Gene Name:	FHIT
UniProt Entry Name:	FHIT_HUMAN

	Western blot analysis of extracts of various cell lines, using FHIT antibody (CAB1196) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat heart using FHIT antibody (CAB1196) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using FHIT antibody (CAB1196) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-FHIT Antibody (CAB9072)	FHIT Colorimetric Cell-Based ELISA Kit
FHIT Antibody (PACO03273)
Phospho-FHIT (Y114) Antibody (PACO06003)
FHIT Antibody (PACO06004)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)