Anti-FLNA Antibody (CAB0927)
- SKU:
- CAB0927
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-FLNA Antibody |
| Antibody SKU: | CAB0927 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2348-2647 of human FLNA (NP_001104026.1). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Jurkat, HeLa, NIH3T3, 293T, Mouse liver, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2348-2647 of human FLNA (NP_001104026.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NQPA SFAV SLNG AKGA IDAK VHSP SGAL EECY VTEI DQDK YAVR FIPR ENGV YLID VKFN GTHI PGSP FKIR VGEP GHGG DPGL VSAY GAGL EGGV TGNP AEFV VNTS NAGA GALS VTID GPSK VKMD CQEC PEGY RVTY TPMA PGSY LISI KYGG PYHI GGSP FKAK VTGP RLVS NHSL HETS SVFV DSLT KATC APQH GAPG PGPA DASK VVAK GLGL SKAY VGQK SSFT VDCS KAGN NMLL VGVH GPRT PCEE ILVK HVGS RLYS VSYL LKDK GEYT LVVK WGDE HIPG SPYR VVVP |
| Gene ID: | 2316 |
| Uniprot: | P21333 |
| Cellular Location: | Cytoplasm, cell cortex, cytoskeleton |
| Calculated MW: | 280kDa |
| Observed MW: | 315kDa |
| Synonyms: | FLNA, ABP-280, ABPX, CSBS, CVD1, FLN, FLN-A, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, XLVD, XMVD, filamin-A |
| Background: | The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | FLNA: a ubiquitous cytoskeletal protein that promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Plays an essential role in embryonic cell migration. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interactions with filamin A may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking. |
| UniProt Protein Details: | Protein type:Actin-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: Xq28 Cellular Component: cortical cytoskeleton; focal adhesion; extracellular region; actin filament; trans-Golgi network; cytosol; actin cytoskeleton; cell soma; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus Molecular Function:Rho GTPase binding; protein homodimerization activity; Rac GTPase binding; Ral GTPase binding; transcription factor binding; actin filament binding; small GTPase binding; signal transducer activity; protein binding; protein kinase C binding; mu-type opioid receptor binding; glycoprotein binding; SMAD binding Biological Process: actin crosslink formation; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; negative regulation of transcription factor activity; mRNA transcription from RNA polymerase II promoter; receptor clustering; dopamine receptor, adenylate cyclase inhibiting pathway; establishment of protein localization; platelet degranulation; early endosome to late endosome transport; actin cytoskeleton reorganization; positive regulation of transcription factor import into nucleus; cytoplasmic sequestering of protein; epithelial to mesenchymal transition; cilium biogenesis; negative regulation of protein catabolic process; blood coagulation Disease: Terminal Osseous Dysplasia; Fg Syndrome 2; Cardiac Valvular Dysplasia, X-linked; Frontometaphyseal Dysplasia; Melnick-needles Syndrome; Heterotopia, Periventricular, Ehlers-danlos Variant; Intestinal Pseudoobstruction, Neuronal, Chronic Idiopathic, X-linked; Heterotopia, Periventricular, X-linked Dominant; Otopalatodigital Syndrome, Type Ii; Otopalatodigital Syndrome, Type I |
| NCBI Summary: | The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009] |
| UniProt Code: | P21333 |
| NCBI GenInfo Identifier: | 116241365 |
| NCBI Gene ID: | 2316 |
| NCBI Accession: | P21333.4 |
| UniProt Secondary Accession: | P21333,Q5HY53, Q5HY55, Q8NF52, E9KL45, |
| UniProt Related Accession: | P21333 |
| Molecular Weight: | 280,739 Da |
| NCBI Full Name: | Filamin-A |
| NCBI Synonym Full Names: | filamin A, alpha |
| NCBI Official Symbol: | FLNAÂ Â |
| NCBI Official Synonym Symbols: | FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280Â Â |
| NCBI Protein Information: | filamin-A; filamin-1; alpha-filamin; non-muscle filamin; actin binding protein 280; endothelial actin-binding protein |
| UniProt Protein Name: | Filamin-A |
| UniProt Synonym Protein Names: | Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin |
| Protein Family: | Filamin |
| UniProt Gene Name: | FLNAÂ Â |
| UniProt Entry Name: | FLNA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FLNA antibody (CAB0927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of HeLa cells using FLNA antibody (CAB0927) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug FLNA antibody (CAB0927). Western blot was performed from the immunoprecipitate using FLNA antibody (CAB0927) at a dilition of 1:1000. |
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