The Phospho-Filamin A-S2152 Antibody (CABP0783) is a high-quality antibody developed for reliable detection and analysis of target proteins. FLNA is a widely expressed actin-binding protein involved in various cellular processes, including cell motility, adhesion, and signaling.This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications to detect and quantify the phosphorylation of FLNA at serine 2152. By specifically binding to the phosphorylated form of FLNA, researchers can gain insights into the signaling pathways and cellular functions regulated by this post-translational modification.The phosphorylation of FLNA at serine 2152 has been implicated in various cellular processes, such as cytoskeletal organization, cell migration, and adhesion dynamics.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-Filamin A-S2152 Antibody
SKU:
CABP0783
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
APSV A
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
2316
RRID
AB_2771116
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-Filamin A-S2152 Rabbit pAb (CABP0783) at 1:1000 dilution. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour . Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Western blot analysis of lysates from HeLa cells, using Phospho-Filamin A-S2152 pAb (CABP0783) at 1:1000 dilution or Filamin A antibody (CAB7896). Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
