Description
FPV NS1 Recombinant Protein (N-His)
FPV NS1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Leu292-Thr584, with an N-terminal His tag. Predicted molecular weight: 35.27 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Initiator protein NS1 is a ~76 kDa protein. Multifunctional protein which displays endonuclease and helicase activities required for initiating and directing viral DNA replication. For research use only.
Also plays a role in viral packaging and transactivation of several promoters. Binds site-specifically to 2-3 approximate tandem copies within the origins of replication (Ori), unwinds this hairpin region and nicks one DNA strand thereby initiating the rolling circle replication (RCR). Cooperatively binds Ori with host PIF and probably other host factors, which activate the nickase function of NS1. Becomes covalently attached to the 5' end of the nick and provides a 3'OH for priming DNA synthesis.
| Product Name: | FPV NS1 Recombinant Protein (N-His) |
| SKU: | GXRP09921 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | FPV |
| Synonyms: | Initiator protein NS1, NS1, EC:3.1.21.-, EC:3.6.4.12, NCVP1, Non-capsid protein NS-1, Non-structural protein 1, Non-structural protein NS1, NS1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Leu292-Thr584 |
| Calculated MW: | 35.27 kDa |
| UniProt: | P24842 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
