Anti-ENO2 Antibody (CAB3118)
- SKU:
- CAB3118
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-ENO2 Antibody |
| Antibody SKU: | CAB3118 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human ENO2 |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, OVCAR3, 22Rv1, SW620, A-549, Jurkat, Mouse brain, Mouse spinal cord, Mouse eye |
| Immunogen: | A synthetic peptide of human ENO2 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 2026 |
| Uniprot: | P09104 |
| Cellular Location: | Cell membrane, Cytoplasm |
| Calculated MW: | 42kDa/47kDa |
| Observed MW: | 50kDa |
| Synonyms: | ENO2, HEL-S-279, NSE, enolase 2 |
| Background: | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. |
| UniProt Protein Function: | ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase Chromosomal Location of Human Ortholog: 12p13 Cellular Component: cytosol; extracellular space; myelin sheath; perikaryon; phosphopyruvate hydratase complex; photoreceptor inner segment; plasma membrane Molecular Function:magnesium ion binding; phosphopyruvate hydratase activity; protein binding Biological Process: carbohydrate metabolic process; gluconeogenesis; glucose metabolic process |
| NCBI Summary: | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P09104 |
| NCBI GenInfo Identifier: | 20981682 |
| NCBI Gene ID: | 2026 |
| NCBI Accession: | P09104.3 |
| UniProt Secondary Accession: | P09104,Q96J33, B7Z2X9, |
| UniProt Related Accession: | P09104 |
| Molecular Weight: | 42,707 Da |
| NCBI Full Name: | Gamma-enolase |
| NCBI Synonym Full Names: | enolase 2 (gamma, neuronal) |
| NCBI Official Symbol: | ENO2Â Â |
| NCBI Official Synonym Symbols: | NSE; HEL-S-279Â Â |
| NCBI Protein Information: | gamma-enolase |
| UniProt Protein Name: | Gamma-enolase |
| UniProt Synonym Protein Names: | 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE |
| Protein Family: | Gamma-enolase |
| UniProt Gene Name: | ENO2Â Â |
| UniProt Entry Name: | ENOG_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ENO2 antibody (CAB3118) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 90S. |
 | Immunohistochemistry of paraffin-embedded rat brain using ENO2 antibody (CAB3118) at dilution of 1:100 (40x lens). |
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