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Metabolism Antibodies 3 Anti-GCLC Antibody CAB4499
Key Features

GCLC Rabbit Monoclonal Antibody (CAB4499)

Datasheet MSDS
SKU CAB4499
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Rat
Host Species Rabbit
Synonyms GCL, GCS, GLCL, GLCLC, GCLC
Research Use Metabolism
€131 - €457

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Description

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GCLC Monoclonal Antibody (CAB4499)

The GCLC Monoclonal Antibody (CAB4499) is a high-quality antibody developed for reliable detection and analysis of target proteins. GCLC is a key enzyme involved in the synthesis of glutathione, an essential antioxidant that plays a critical role in protecting cells from oxidative stress and maintaining cellular health.This high-quality antibody, developed using rabbit monoclonal technology, offers exceptional sensitivity and specificity for detecting GCLC in a variety of samples, including human tissues and cell lines. It has been validated for use in Western blotting, immunohistochemistry, and immunofluorescence applications, providing researchers with multiple options for analyzing GCLC expression and localization.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.

Product Name:GCLC Monoclonal Antibody
SKU:CAB4499
Size:20μL, 100μL
Reactivity:Human, Rat
Clone Number:ARC1065
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:LNSY LENM EVDV DTRC SILN YLKL IKKR ASGE LMTV ARWM REFI ANHP DYKQ DSVI TDEM NYSL ILKC NQIA NELC ECPE LLGS AFRK VKYS GSKT DSSN
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:100 - 1:800
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:GCL, GCS, GLCL, GLCLC, GCLC
Positive Sample:HeLa, HepG2, Rat kidney
Cellular Localization:Cytosol, Glutamate-Cysteine Ligase Complex, Mitochondrion.
Calculated MW:73kDa
Observed MW:73kDa

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Purification MethodAffinity purification
Gene ID2729
RRIDAB_2863282
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Rat kidney, using GCLC Rabbit mAb (CAB4499) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Product ImageWestern blot analysis of various lysates using GCLC Rabbit mAb (CAB4499) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
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