The General Cortisol Cor ELISA Kit from Assay Genie is a cutting-edge tool for measuring cortisol levels in a variety of biological samples. Whether analyzing serum, plasma, or cell culture supernatants, this kit provides researchers with precise and reliable results, thanks to its high sensitivity and specificity.Cortisol, often referred to as the "stress hormone," plays a crucial role in the body's response to stress and regulation of various physiological processes. Imbalances in cortisol levels have been associated with conditions such as anxiety, depression, and obesity, highlighting the importance of accurate measurement in research and clinical settings.
With the General Cortisol Cor ELISA Kit, scientists can gain valuable insights into the role of cortisol in health and disease, paving the way for innovative approaches to treatment and management of cortisol-related disorders. Trust Assay Genie for quality and performance in cortisol testing.
Product Name:
General Cortisol (Cor) ELISA Kit
Product Code:
UNDL00023
Size:
96 Assays
Target:
Cortisol
Synonyms:
Hydrocortisone
Detection Method:
Competition
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
90-100
95
Heparin plasma (n=5)
90-102
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
