The General Noradrenaline (NE) ELISA Kit from AssayGenie is specifically designed for the accurate detection of noradrenaline levels in various biological samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.Noradrenaline, also known as norepinephrine, is a key neurotransmitter and hormone involved in the body's stress response. It plays a crucial role in regulating blood pressure, heart rate, and the body's fight or flight response. Abnormal levels of noradrenaline have been linked to various disorders, including hypertension, anxiety, and depression, making it an important biomarker for studying these conditions and developing potential therapies.
Our General Noradrenaline (NE) ELISA Kit provides researchers with a valuable tool for studying the role of noradrenaline in health and disease. With its high sensitivity and accuracy, this kit allows for precise measurement of noradrenaline levels in biological samples, ultimately advancing our understanding of this critical neurotransmitter and its impact on human health.
Product Name:
General Noradrenaline (NE) ELISA Kit
Product Code:
UNDL00055
Size:
96 Assays
Target:
NE
Synonyms:
NA, NAd, Norepinephrine, Norepi
Detection Method:
Competition
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
