The General Prostaglandin E2 (PGE2) ELISA Kit from Assay Genie is a powerful tool for accurately measuring levels of prostaglandin E2 in various biological samples. Prostaglandin E2 is a key inflammatory mediator that plays a crucial role in a wide range of physiological processes, including inflammation, pain, and fever.This ELISA kit offers high sensitivity and specificity, ensuring precise and reliable results every time. It is compatible with samples such as serum, plasma, and cell culture supernatants, making it versatile for a variety of research applications.
Prostaglandin E2 is known to be involved in various diseases and conditions, such as arthritis, cancer, and cardiovascular diseases. By measuring levels of PGE2, researchers can gain valuable insights into the pathophysiology of these conditions and potentially identify new therapeutic targets. Overall, the General Prostaglandin E2 (PGE2) ELISA Kit is a valuable tool for studying the role of prostaglandin E2 in health and disease, making it a must-have for any laboratory conducting research in the field of inflammation and related disorders.
Product Name:
General Prostaglandin E2 (PGE2) ELISA Kit
Product Code:
UNDL00063
Size:
96 Assays
Target:
PGE2
Synonyms:
PG-E2
Detection Method:
Competition
Range:
4.687-300pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
