The PGE2 (Prostaglandin E2) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of PGE2 levels in various biological samples such as serum, plasma, and cell culture supernatants. This kit ensures reliable and reproducible results, making it suitable for a wide range of research applications.Prostaglandin E2 is a key mediator of inflammation, pain, and fever, playing a crucial role in a variety of physiological and pathological processes. By measuring PGE2 levels, researchers can gain valuable insights into the mechanisms underlying inflammatory diseases, cancer, and other health conditions, paving the way for the development of novel therapeutic strategies. With its user-friendly protocol and excellent performance characteristics, the PGE2 ELISA Kit from Assay Genie is an indispensable tool for studying the role of prostaglandins in health and disease. Get accurate and dependable results with this cutting-edge assay kit.
Product Name:
PGE2 (Prostaglandin E2) ELISA Kit
SKU:
UNES00024
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
