The General Thromboxane B2 (TXB2) ELISA Kit from AssayGenie is a reliable and accurate tool for the detection of thromboxane B2 levels in various samples including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides consistent and reproducible results, making it an essential tool for researchers in various fields.Thromboxane B2 is a metabolite of thromboxane A2, a potent vasoconstrictor and platelet aggregator that plays a crucial role in various physiological processes including blood clotting and inflammation. Dysregulation of thromboxane B2 levels has been associated with several health conditions such as cardiovascular diseases, asthma, and inflammatory disorders, highlighting the importance of monitoring its levels in biological samples.
With the General Thromboxane B2 (TXB2) ELISA Kit, researchers can accurately quantify thromboxane B2 levels in their samples, enabling them to better understand the role of this bioactive lipid mediator in disease pathogenesis and potentially identify novel therapeutic targets. Trust AssayGenie's ELISA kits for reliable and reproducible results in your research endeavors.
Product Name:
General Thromboxane B2 (TXB2) ELISA Kit
Product Code:
UNDL00076
Size:
96 Assays
Target:
TXB2
Synonyms:
TX-B2
Detection Method:
Competition
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
