Description
GenieKine Recombinant Human G-CSF/CSF3 Protein (GKRP0045)
GenieKine Recombinant Human G-CSF/CSF3 Protein is expressed in E. coli. G-CSF stimulates the proliferation and differentiation of neutrophil progenitors and the release of neutrophils from bone marrow. Supplied as a lyophilized powder with purity of >97% by SDS-PAGE and endotoxin below 0.02 EU/µg by LAL assay. Suitable for immune cell culture and stem cell culture. For research use only.
This product (SKU: GKRP0045) is expressed in E. coli and is supplied unconjugated. It has a calculated molecular weight of 18.7 kDa (monomer) and an observed molecular weight of 17 kDa (reducing) on SDS-PAGE. Purity is >97% by SDS-PAGE and endotoxin is <0.02 EU/µg by the LAL method.
Key Features
| High Purity: >97% by SDS-PAGE | |
| Low Endotoxin: <0.02 EU/µg | |
| Bioactivity Validated: ED50 10-50 pg/mL | |
| Expressed in E. coli |
| Product Name: | GenieKine Recombinant Human G-CSF/CSF3 Protein |
| SKU: | GKRP0045 |
| Size: | 2 µg, 10 µg, 50 µg, 100 µg, 250 µg, 500 µg, 1 mg |
| Reactivity: | Human |
| Synonyms: | Granulocyte Colony Stimulating Factor, Colony Stimulating Factor 3GCSF, CSF3, C17orf33, CSF3OS, Filgrastim, Pluripoietin, Lenograstim |
| Tag: | Unconjugated |
| Calculated MW: | 18.7 kDa (monomer) |
| Observed MW: | 17 kDa (reducing) |
| Gene ID: | 1440 |
| UniProt: | P09919 |
| Expression System: | E. coli |
| Sequence: | Thr31-Pro204 |
| Biological Activity: | Determined by a cell proliferation assay using mouse M-NFS-60 cells. The ED50 for this effect is typically 10-50 pg/mL. |
| Endotoxin: | <0.02 EU/µg of protein, as determined by the LAL method. |
| Purity: | >97% by SDS-PAGE and quantitative densitometry by Coomassie Blue staining. |
| Formulation: | Lyophilized from sterile Sodium Acetate and Sodium Chloride with Trehalose. |
| Reconstitution: | Reconstitute at 100 µg/mL in sterile PBS. |
| Storage: | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
G-CSF primarily stimulates the proliferation, differentiation, and survival of neutrophilic granulocyte precursors in the bone marrow and stimulates upregulation of mature neutrophil release into the bloodstream as part of the innate immune response. During an immune response, G-CSF is secreted by monocytes, macrophages, and activated T cells. G-CSF binds to the G-CSF receptor (G-CSFR), which is expressed on the surface of hematopoietic cells, particularly myeloid progenitors. G-CSF signaling occurs via the JAK/STAT, Ras/MAPK and PI3K/Akt pathways.
 | Determined by a cell proliferation assay using mouse M-NFS-60 cells. The ED50 for this effect is typically 10-50 pg/mL. |
 | Recombinant Human G-CSF (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie Blue showing a single band at approximately 17 kDa and purity greater than 97%. |
