Anti-GLDC Antibody (CAB9933)

Antibody Name:	Anti-GLDC Antibody
Antibody SKU:	CAB9933
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 36-290 of human GLDC (NP_000161.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, DU145

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 36-290 of human GLDC (NP_000161.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DSSS GGGD SAAA GASR LLER LLPR HDDF ARRH IGPG DKDQ REML QTLG LASI DELI EKTV PANI RLKR PLKM EDPV CENE ILAT LHAI SSKN QIWR SYIG MGYY NCSV PQTI LRNL LENS GWIT QYTP YQPE VSQG RLES LLNY QTMV CDIT GLDM ANAS LLDE GTAA AEAL QLCY RHNK RRKF LVDP RCHP QTIA VVQT RAKY TGVL TELK LPCE MDFS GKDV SGVL FQYP DTEG KVED FTEL VERA HQSG SLA
Gene ID:	2731
Uniprot:	P23378
Cellular Location:	Mitochondrion
Calculated MW:	112kDa
Observed MW:	113kDa

Synonyms:

GLDC, GCE, GCSP, HYGN1

Background:

Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).

UniProt Protein Function:	GLDC: The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. Defects in GLDC are a cause of non-ketotic hyperglycinemia (NKH); also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms. Belongs to the GcvP family.
UniProt Protein Details:	Protein type:EC 1.4.4.2; Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine Chromosomal Location of Human Ortholog: 9p22 Cellular Component: cytosol; glycine cleavage complex; mitochondrial matrix; mitochondrion; nucleus; plasma membrane Molecular Function:electron carrier activity; glycine binding; glycine dehydrogenase (decarboxylating) activity Biological Process: glycine catabolic process; glycine decarboxylation via glycine cleavage system; glyoxylate metabolic process Disease: Glycine Encephalopathy
NCBI Summary:	Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).[provided by RefSeq, Jan 2010]
UniProt Code:	P23378
NCBI GenInfo Identifier:	229462870
NCBI Gene ID:	2731
NCBI Accession:	P23378.2
UniProt Secondary Accession:	P23378,Q2M2F8,
UniProt Related Accession:	P23378
Molecular Weight:	112,730 Da
NCBI Full Name:	Glycine dehydrogenase (decarboxylating), mitochondrial
NCBI Synonym Full Names:	glycine decarboxylase
NCBI Official Symbol:	GLDC
NCBI Official Synonym Symbols:	GCE; GCSP; HYGN1
NCBI Protein Information:	glycine dehydrogenase (decarboxylating), mitochondrial
UniProt Protein Name:	Glycine dehydrogenase (decarboxylating), mitochondrial
UniProt Synonym Protein Names:	Glycine cleavage system P protein; Glycine decarboxylase; Glycine dehydrogenase (aminomethyl-transferring)
UniProt Gene Name:	GLDC
UniProt Entry Name:	GCSP_HUMAN

	Western blot analysis of extracts of various cell lines, using GLDC antibody (CAB9933) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunoprecipitation analysis of 200ug extracts of DU145 cells using 3ug GLDC antibody (CAB9933). Western blot was performed from the immunoprecipitate using GLDC antibody (CAB9933) at a dilition of 1:1000.

ELISA Kits

Human GLDC / GlycineÂ Dehydrogenase ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)