Anti-GLS Antibody (CAB3885)

Antibody Name:	Anti-GLS Antibody
Antibody SKU:	CAB3885
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 610-669 of human GLS (NP_055720.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, NIH/3T3, Mouse brain, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 610-669 of human GLS (NP_055720.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KVNP FPKD RWNN TPMD EALH FGHH DVFK ILQE YQVQ YTPQ GDSD NGKE NQTV HKNL DGLL
Gene ID:	2744
Uniprot:	O94925
Cellular Location:	Cytoplasm, Mitochondrion, cytosol
Calculated MW:	17kDa/65kDa/73kDa
Observed MW:	65-73KDa

Synonyms:

GLS, AAD20, GAC, GAM, GLS1, KGA

Background:

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	glutaminase: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. Belongs to the glutaminase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - arginine and proline; Hydrolase; Mitochondrial; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Energy Metabolism - nitrogen; EC 3.5.1.2 Chromosomal Location of Human Ortholog: 2q32-q34 Cellular Component: mitochondrion; mitochondrial matrix; cytosol Molecular Function:protein binding; glutaminase activity Biological Process: synaptic transmission; glutamate secretion; suckling behavior; neurotransmitter secretion; glutamine catabolic process; neurological control of breathing; glutamate biosynthetic process; amino acid biosynthetic process; protein homotetramerization
NCBI Summary:	This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
UniProt Code:	O94925
NCBI GenInfo Identifier:	12643334
NCBI Gene ID:	2744
NCBI Accession:	O94925.1
UniProt Related Accession:	O94925
Molecular Weight:	65; 73kDa
NCBI Full Name:	Glutaminase kidney isoform, mitochondrial
NCBI Synonym Full Names:	glutaminase
NCBI Official Symbol:	GLS
NCBI Official Synonym Symbols:	GAC; GAM; KGA; GLS1; AAD20; GDPAG; CASGID; EIEE71
NCBI Protein Information:	glutaminase kidney isoform, mitochondrial
UniProt Protein Name:	Glutaminase kidney isoform, mitochondrial
UniProt Synonym Protein Names:	K-glutaminase; L-glutamine amidohydrolase
Protein Family:	Glutaminase
UniProt Gene Name:	GLS
UniProt Entry Name:	GLSK_HUMAN

	Western blot analysis of extracts of various cell lines, using GLS antibody (CAB3885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human stomach using GLS antibody (CAB3885) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using GLS antibody (CAB3885) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-Glutaminase Antibody (CAB11043)	Human GLS / Glutaminase kidney isoform, mitochondrial ELISA Kit
GLS Antibody (PACO09489)	Human Glutaminase kidney isoform, mitochondrial (GLS) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)