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Glycogen Fluorometric Assay Kit (MAES0011)

SKU:
MAES0011
Product Type:
Assay
Detection Method:
Fluorometric
Instrument:
Microplate Reader
Sample Type:
Liver Tissue, Muscle Tissue
Sensitivity:
0.06 ug/mL
Range:
0.06-4.0 ug/mL
Assay Time:
50min
Shipping:
Gel Pack
Research Area:
Glycolysis & Carbohydrates
Diabetes & Obesity
Food Safety & Analysis
Clinical Chemistry
$695
Frequently bought together:

Description

system_update_altDatasheet
Product Name:Glycogen Fluorometric Assay Kit
Product Code:MAES0011
Product Size:96 Assays
Sample Type:Animal liver and muscle tissue
Assay Time:50min
Instrument:Fluorescence microplate reader
Sensitivity:0.06 µg/mL
Detection Range:0.06-4.0 µg/mL
Inter CV:6.6
Inter CV:3.4

Glycogen is mainly produced by liver and muscle. Glycogen content is mainly regulated by glycogen synthase and glycogen phosphorylase. In the liver, glycogen acts as a glucose store for other tissues and maintains blood glucose levels. In muscle, glycogen is mainly used as energy for the supply of adenosine triphosphate (ATP) during muscle contraction. Due to the lack of glucose-6-phosphatase, muscle glycogen cannot maintain the level blood glucose.

Glycogen produces glucose under the action of starch glycosidase, and glucose is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of the peroxidase, hydrogen peroxide be oxidized to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm is proportional to the glycogen content.

Shelf Life:6 Months
Storage:-20°C
Shipping:Gel Pack
Research Area:Glycolysis & Carbohydrates

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