The GNB2 Antibody (CAB8422) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific to human samples, making it ideal for Western blot applications. By binding to GNB2, this antibody enables accurate detection and analysis of GNB2 protein levels in various cell types.GNB2 is a key player in intracellular signal transduction, particularly in processes involving G protein-coupled receptors. Dysregulation of GNB2 signaling has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GNB2 Antibody
SKU:
CAB8422
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SSS4, HG2C1, NEDHYDF, GNB2
Positive Sample:
HeLa, A-431, BT-474, Mouse brain, Mouse lung, Rat brain
Cellular Localization:
Cytoplasm, Perinuclear Region.
Calculated MW:
37kDa
Observed MW:
37kDa
Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene contains a trinucleotide (CCG) repeat length polymorphism in its 5' UTR.
Purification Method
Affinity purification
Gene ID
2783
RRID
AB_2769637
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GNB2 Rabbit pAb (CAB8422) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat stomach using GNB2 Rabbit pAb (CAB8422) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using GNB2 Rabbit pAb (CAB8422) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of L929 cells using GNB2 Rabbit pAb (CAB8422) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
