Anti-GORASP1 Antibody (CAB6609)

Antibody Name:	Anti-GORASP1 Antibody
Antibody SKU:	CAB6609
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 221-440 of human GORASP1 (NP_114105.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human
Positive Samples:	BT474, U-937

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 221-440 of human GORASP1 (NP_114105.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ALPL GAPP PDAL PPGP TPED SPSL ETGS RQSD YMEA LLQA PGSS MEDP LPGP GSPS HSAP DPDG LPHF METP LQPP PPVQ RVMD PGFL DVSG ISLL DNSN ASVW PSLP SSTE LTTT AVST SGPE DICS SSSS HERG GEAT WSGS EFEV SFLD SPGA QAQA DHLP QLTL PDSL TSAA SPED GLSA ELLE AQAE EEPA STEG LDTG TEAE GLDS QAQI STTE
Gene ID:	64689
Uniprot:	Q9BQQ3
Cellular Location:	Cytoplasmic side, Golgi apparatus, Peripheral membrane protein, cis-Golgi network membrane
Calculated MW:	23kDa/26kDa/46kDa
Observed MW:	65kDa

Synonyms:

GORASP1, GOLPH5, GRASP65, P65

Background:

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene.

UniProt Protein Function:	GRASP65: Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes. Belongs to the GORASP family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 3p22-p21.33 Cellular Component: Golgi membrane; Golgi apparatus Biological Process: protein transport; negative regulation of dendrite morphogenesis; mitotic cell cycle; Golgi organization and biogenesis
NCBI Summary:	The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
UniProt Code:	Q9BQQ3
NCBI GenInfo Identifier:	51316077
NCBI Gene ID:	64689
NCBI Accession:	Q9BQQ3.3
UniProt Related Accession:	Q9BQQ3
Molecular Weight:
NCBI Full Name:	Golgi reassembly-stacking protein 1
NCBI Synonym Full Names:	golgi reassembly stacking protein 1
NCBI Official Symbol:	GORASP1
NCBI Official Synonym Symbols:	P65; GOLPH5; GRASP65
NCBI Protein Information:	Golgi reassembly-stacking protein 1
UniProt Protein Name:	Golgi reassembly-stacking protein 1
UniProt Synonym Protein Names:	Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; GOLPH5; Golgi reassembly-stacking protein of 65 kDa; GRASP65
UniProt Gene Name:	GORASP1
UniProt Entry Name:	GORS1_HUMAN

	Western blot analysis of extracts of various cell lines, using GORASP1 antibody (CAB6609) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human gastric cancer using GORASP1 antibody (CAB6609) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using GORASP1 antibody (CAB6609). Blue: DAPI for nuclear staining.

ELISA Kits

Human GORASP1 (Golgi reassembly-stacking protein 1) ELISA Kit (HUFI04046)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)