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Anti-GZMA Antibody (CAB6231)

SKU:
CAB6231
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
  • Cell Death Antibodies 2 Anti-GZMA Antibody CAB6231
  • Cell Death Antibodies 2 Anti-GZMA Antibody CAB6231
€139 - €419
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Description

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Antibody Name:Anti-GZMA Antibody
Antibody SKU:CAB6231
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 29-262 of human GZMA (NP_006135.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse spleen, Mouse thymus, Rat intestine, Rat spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 29-262 of human GZMA (NP_006135.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IIGG NEVT PHSR PYMV LLSL DRKT ICAG ALIA KDWV LTAA HCNL NKRS QVIL GAHS ITRE EPTK QIML VKKE FPYP CYDP ATRE GDLK LLQL TEKA KINK YVTI LHLP KKGD DVKP GTMC QVAG WGRT HNSA SWSD TLRE VNIT IIDR KVCN DRNH YNFN PVIG MNMV CAGS LRGG RDSC NGDS GSPL LCEG VFRG VTSF GLEN KCGD PRGP GVYI LLSK KHLN WIIM TIKG AV
Gene ID:3001
Uniprot:P12544
Cellular Location:Cytoplasmic granule, Secreted
Calculated MW:27kDa/28kDa
Observed MW:35kDa
Synonyms:GZMA, CTLA3, HFSP
Background:Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells.
UniProt Protein Function:GZMA: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis. Homodimer; disulfide-linked. Interacts with APEX1. Dexamethasone (DEX) induces expression of isoform beta and represses expression of isoform alpha. The alteration in expression is mediated by binding of glucocorticoid receptor to independent promoters adjacent to the alternative first exons of isoform alpha and isoform beta. Belongs to the peptidase S1 family. Granzyme subfamily. 2 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Protease; EC 3.4.21.78; Apoptosis; Secreted

Chromosomal Location of Human Ortholog: 5q11-q12

Cellular Component: extracellular region; immunological synapse; nucleus

Molecular Function:protein binding; protein homodimerization activity; serine-type endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; positive regulation of apoptosis; apoptosis; cytolysis; negative regulation of oxidoreductase activity; negative regulation of endodeoxyribonuclease activity; immune response; negative regulation of DNA binding

NCBI Summary:Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008]
UniProt Code:P12544
NCBI GenInfo Identifier:317373360
NCBI Gene ID:3001
NCBI Accession:P12544.2
UniProt Secondary Accession:P12544,Q6IB36, A4PHN1,
UniProt Related Accession:P12544
Molecular Weight:262
NCBI Full Name:Granzyme A
NCBI Synonym Full Names:granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
NCBI Official Symbol:GZMA  
NCBI Official Synonym Symbols:HFSP; CTLA3  
NCBI Protein Information:granzyme A; HF; h factor; CTL tryptase; fragmentin-1; cytotoxic T-lymphocyte proteinase 1; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease)
UniProt Protein Name:Granzyme A
UniProt Synonym Protein Names:CTL tryptase; Cytotoxic T-lymphocyte proteinase 1; Fragmentin-1; Granzyme-1; Hanukkah factor; H factor; HF
Protein Family:Granzyme
UniProt Gene Name:GZMA  
UniProt Entry Name:GRAA_HUMAN
Anti-GZMA Antibody (CAB6231)Western blot analysis of extracts of various cell lines, using GZMA antibody (CAB6231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-GZMA Antibody (CAB6231)Immunofluorescence analysis of U2OS cells using GZMA antibody (CAB6231) at dilution of 1:100. Blue: DAPI for nuclear staining.

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