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Anti-GRB14 Antibody (CAB2742)

SKU:
CAB2742
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-GRB14 Antibody
Antibody SKU:CAB2742
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human GRB14
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:25 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Human fetal intestine
Immunogen:Recombinant protein of human GRB14
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:2888
Uniprot:Q14449
Cellular Location:Cytoplasm, Endosome membrane, Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:51kDa/60kDa
Observed MW:61kDa
Synonyms:GRB14
Background:The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:GRB14: Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal. Belongs to the GRB7/10/14 family.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2q22-q24

Cellular Component: Golgi membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; endosome membrane; cytosol

Molecular Function:SH3/SH2 adaptor activity

Biological Process: positive regulation of signal transduction; signal transduction; blood coagulation; leukocyte migration

NCBI Summary:The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Code:Q14449
NCBI GenInfo Identifier:59802920
NCBI Gene ID:2888
NCBI Accession:Q14449.2
UniProt Secondary Accession:Q14449,Q7Z6I1, B7Z7F9,
UniProt Related Accession:Q14449
Molecular Weight:540
NCBI Full Name:Growth factor receptor-bound protein 14
NCBI Synonym Full Names:growth factor receptor-bound protein 14
NCBI Official Symbol:GRB14  
NCBI Protein Information:growth factor receptor-bound protein 14; GRB14 adapter protein
UniProt Protein Name:Growth factor receptor-bound protein 14
UniProt Synonym Protein Names:GRB14 adapter protein
Protein Family:Growth factor receptor-bound protein
UniProt Gene Name:GRB14  
UniProt Entry Name:GRB14_HUMAN
Anti-GRB14 Antibody (CAB2742)Western blot analysis of extracts of human fetal intestine, using GRB14 antibody (CAB2742). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

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