The GRHL3 Polyclonal Antibody (PAC049258) is a valuable tool for researchers studying the GRHL3 protein, a transcription factor involved in epithelial cell differentiation and development. This antibody is raised in rabbits and is highly specific for human samples, making it ideal for a variety of experimental techniques, including Western blotting.GRHL3 is known for its role in regulating gene expression and cell differentiation in various tissues, including the skin, hair follicles, and lungs.
Dysregulation of GRHL3 has been implicated in diseases such as cancer, developmental disorders, and certain skin conditions. By using this antibody, researchers can investigate the function of GRHL3 in normal and diseased tissues, providing insights into potential therapeutic targets for a range of conditions.
Antibody Name:
GRHL3 Antibody (PACO49258)
Antibody SKU:
PACO49258
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Grainyhead-like protein 3 homolog protein (31-182AA)
Immunofluorescent analysis of PC-3 cells using PACO49258 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Transcription factor playing important roles in primary neurulation and in the differentiation of stratified epithelia of both ectodermal and endodermal origin (By similarity). Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes. xhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair (By similarity). Exhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair but is essential to form the epidermal barrier with TGM3 as critical direct target gene among others. Despite being dispensable during normal epidermal homeostasis in the adulthood, is again required for barrier repair after immune-mediated epidermal damage, regulates distinct gene batteries in embryonic epidermal differentiation and adult epidermal barrier reformation after injury. Plays unique and cooperative roles with GRHL2 in establishing distinct zones of primary neurulation. Essential for spinal closure, functions cooperatively with GRHL2 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (By similarity). Also required for proper development of the oral periderm. No genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity.
Synonyms:
Grainyhead-like protein 3 homolog (Sister of mammalian grainyhead) (Transcription factor CP2-like 4), GRHL3, SOM TFCP2L4
UniProt Protein Function:
GRHL3: May function as a transcription factor. Belongs to the grh/CP2 family. Grainyhead subfamily. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Transcription factor; DNA-bindingChromosomal Location of Human Ortholog: 1p36.11Cellular Component: cytoplasm; nucleoplasm; nucleusMolecular Function: chromatin DNA binding; protein binding; sequence-specific DNA bindingBiological Process: epidermis development; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; regulation of actin cytoskeleton organization and biogenesisDisease: Van Der Woude Syndrome 2
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the grainyhead family of transcription factors. The encoded protein may function as a transcription factor during development, and has been shown to stimulate migration of endothelial cells. Multiple transcript variants encoding distinct isoforms have been identified for this gene.[provided by RefSeq, Aug 2010]
