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Anti-GRK6 Antibody (CAB6379)

SKU:
CAB6379
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-GRK6 Antibody CAB6379
  • Metabolism Antibodies 2 Anti-GRK6 Antibody CAB6379
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-GRK6 Antibody
Antibody SKU:CAB6379
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human GRK6 (NP_001004106.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, Jurkat, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human GRK6 (NP_001004106.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MELE NIVA NTVL LKAR EGGG GNRK GKSK KWRQ MLQF PHIS QCEE LRLS LERD YHSL CERQ PIGR LLFR EFCA TRPE LSRC VAFL DGVA EYEV TPDD KRKA CGRQ LTQN FLSH TGPD LIPE VPRQ LVTN CTQR LEQG PCKD LFQE LTRL THEY LSVA PFAD YLDS IYFN RF
Gene ID:2870
Uniprot:P43250
Cellular Location:Lipid-anchor, Membrane
Calculated MW:64kDa/65kDa/67kDa
Observed MW:66kDa
Synonyms:GRK6, GPRK6
Background:This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene.
UniProt Protein Function:GRK6: Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamin receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro). Widely expressed. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.7.11.16; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; GRK family; GRK subfamily

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: membrane

Molecular Function:G-protein coupled receptor kinase activity; protein binding; ATP binding

Biological Process: Wnt receptor signaling pathway; protein amino acid phosphorylation; regulation of G-protein coupled receptor protein signaling pathway

NCBI Summary:This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P43250
NCBI GenInfo Identifier:20141386
NCBI Gene ID:2870
NCBI Accession:P43250.2
UniProt Secondary Accession:P43250,O60541, Q13652,
UniProt Related Accession:P43250
Molecular Weight:64,294 Da
NCBI Full Name:G protein-coupled receptor kinase 6
NCBI Synonym Full Names:G protein-coupled receptor kinase 6
NCBI Official Symbol:GRK6  
NCBI Official Synonym Symbols:GPRK6  
NCBI Protein Information:G protein-coupled receptor kinase 6
UniProt Protein Name:G protein-coupled receptor kinase 6
UniProt Synonym Protein Names:G protein-coupled receptor kinase GRK6
Protein Family:G protein-coupled receptor kinase
UniProt Gene Name:GRK6  
UniProt Entry Name:GRK6_HUMAN
Anti-GRK6 Antibody (CAB6379)Western blot analysis of extracts of various cell lines, using GRK6 antibody (CAB6379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-GRK6 Antibody (CAB6379)Immunofluorescence analysis of MCF7 cells using GRK6 antibody (CAB6379). Blue: DAPI for nuclear staining.

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