Anti-GRM1 Antibody (CAB11094)

Antibody Name:	Anti-GRM1 Antibody
Antibody SKU:	CAB11094
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 921-1010 of human GRM1 (NP_001264993.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Rat
Positive Samples:	Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 921-1010 of human GRM1 (NP_001264993.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ETAC NQTA VIKP LTKS YQGS GKSL TFSD TSTK TLYN VEEE EDAQ PIRF SPPG SPSM VVHR RVPS AATT PPLP SHLT AEET PLFL AEPA LP
Gene ID:	2911
Uniprot:	Q13255
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	101kDa/132kDa
Observed MW:	132kDa

Synonyms:

GRM1, GPRC1A, MGLU1, MGLUR1, PPP1R85, SCAR13

Background:

This gene encodes a metabotropic glutamate receptor that functions by activating phospholipase C. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The canonical alpha isoform of the encoded protein is a disulfide-linked homodimer whose activity is mediated by a G-protein-coupled phosphatidylinositol-calcium second messenger system. This gene may be associated with many disease states, including schizophrenia, bipolar disorder, depression, and breast cancer. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	mGluR1: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol- calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. Homodimer; disulfide-linked. The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3 and GRASP. Belongs to the G-protein coupled receptor 3 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:GPCR, family 3; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: 6q24.3 Cellular Component: integral to plasma membrane; plasma membrane; postsynaptic density; presynaptic membrane Molecular Function:G-protein coupled receptor activity; glutamate receptor activity; protein binding Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); G-protein coupled receptor protein signaling pathway; metabotropic glutamate receptor signaling pathway; metabotropic glutamate receptor, adenylate cyclase inhibiting pathway; regulation of synaptic transmission, glutamatergic; synaptic transmission Disease: Spinocerebellar Ataxia, Autosomal Recessive 13
NCBI Summary:	This gene encodes a metabotropic glutamate receptor that functions by activating phospholipase C. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The canonical alpha isoform of the encoded protein is a disulfide-linked homodimer whose activity is mediated by a G-protein-coupled phosphatidylinositol-calcium second messenger system. This gene may be associated with many disease states, including schizophrenia, bipolar disorder, depression, and breast cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]
UniProt Code:	Q13255
NCBI GenInfo Identifier:	311033443
NCBI Gene ID:	2911
NCBI Accession:	Q13255.3
UniProt Secondary Accession:	Q13255,Q13256, Q14757, Q14758, Q5VTF7, Q5VTF8, Q9NU10 Q9UGS9, Q9UGT0, B9EG79, F8W805,
UniProt Related Accession:	Q13255
Molecular Weight:
NCBI Full Name:	Metabotropic glutamate receptor 1
NCBI Synonym Full Names:	glutamate metabotropic receptor 1
NCBI Official Symbol:	GRM1
NCBI Official Synonym Symbols:	MGLU1; GPRC1A; MGLUR1; SCAR13; PPP1R85
NCBI Protein Information:	metabotropic glutamate receptor 1
UniProt Protein Name:	Metabotropic glutamate receptor 1
Protein Family:	Metabotropic glutamate receptor
UniProt Gene Name:	GRM1

	Western blot analysis of extracts of rat liver, using GRM1 antibody (CAB11094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of C6 cells using GRM1 Rabbit pAb (CAB11094) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-GRM1 Antibody (CAB11462)

Anti-GRM1 Antibody (CAB2963)

GRM1 Antibody (PACO03990)

GRM1 Antibody (PACO09596)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)