Anti-GRP Antibody (CAB6380)

Antibody Name:	Anti-GRP Antibody
Antibody SKU:	CAB6380
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human GRP (NP_002082.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung, Mouse spinal cord, Rat brain, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human GRP (NP_002082.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRGR ELPL VLLA LVLC LAPR GRAV PLPA GGGT VLTK MYPR GNHW AVGH LMGK KSTG ESSS VSER GSLK QQLR EYIR WEEA ARNL LGLI EAKE NRNH QPPQ PKAL GNQQ PSWD SEDS SNFK DVGS KGKV GRLS APGS QREG RNPQ LNQQ
Gene ID:	2922
Uniprot:	P07492
Cellular Location:	Secreted
Calculated MW:	15kDa/16kDa
Observed MW:	16kDa

Synonyms:

GRP, BN, GRP-10, preproGRP, proGRP, GRP

Background:

This gene encodes a member of the bombesin-like family of gastrin-releasing peptides. The encoded preproprotein is proteolytically processed to generate two peptides, gastrin-releasing peptide and neuromedin-C. These peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

UniProt Protein Function:	GRP: GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP- receptor gene expression, long-term potentiation, and amygdala- dependent memory for fear. Belongs to the bombesin/neuromedin-B/ranatensin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 18q21.1-q21.32 Cellular Component: extracellular space; extracellular region Molecular Function:neuropeptide hormone activity; receptor binding Biological Process: neuropeptide signaling pathway; signal transduction
NCBI Summary:	This gene encodes a member of the bombesin-like family of gastrin-releasing peptides. Its preproprotein, following cleavage of a signal peptide, is further processed to produce either the 27 aa gastrin-releasing peptide or the 10 aa neuromedin C. These smaller peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	P07492
NCBI GenInfo Identifier:	308153451
NCBI Gene ID:	2922
NCBI Accession:	P07492.2
UniProt Secondary Accession:	P07492,P07491, P81553, Q14454, Q53YA0, Q9BSY7,
UniProt Related Accession:	P07492
Molecular Weight:	148
NCBI Full Name:	Gastrin-releasing peptide
NCBI Synonym Full Names:	gastrin-releasing peptide
NCBI Official Symbol:	GRP
NCBI Official Synonym Symbols:	BN; GRP-10; proGRP; preproGRP
NCBI Protein Information:	gastrin-releasing peptide; bombesin; prepro-GRP; neuromedin C; pre-progastrin releasing peptide
UniProt Protein Name:	Gastrin-releasing peptide
UniProt Synonym Protein Names:	GRP-10
Protein Family:	GrpE protein
UniProt Gene Name:	GRP
UniProt Entry Name:	GRP_HUMAN

	Western blot analysis of extracts of various cell lines, using GRP antibody (CAB6380) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using GRP antibody (CAB6380) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
GRP Antibody (PACO09598)	Human Gastrin-releasing peptide (GRP) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)