Western blot analysis of lysates from HT-29+NK-92, using Cleaved Gasdermin B Rabbit pAb (CAB23046) at 1:400 dilution. HT-29 and NK-92 cells were treated by IFNγ (50ng/ml) for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features
Cleaved Gasdermin B Polyclonal Antibody (CAB23046)
Cleaved Gasdermin B Polyclonal Antibody (CAB23046)
The Cleaved Gasdermin B Polyclonal Antibody (CAB23046) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for cleaved Gasdermin B and is validated for use in various experimental techniques, including Western blot analysis.Gasdermin B is involved in the formation of pores in cell membranes during pyroptosis, leading to cell lysis and release of pro-inflammatory cytokines.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Cleaved Gasdermin B Polyclonal Antibody
SKU:
CAB23046
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GSDML, PP4052, GSDMB-1, PRO2521, Cleaved Gasdermin B
Positive Sample:
Co-culture of HT-29 and NK-92 treated with IFNγ
Calculated MW:
18kDa/45kDa/46kDa/47kDa
Observed MW:
16kDa
This gene encodes a member of the gasdermin-domain containing protein family. Other gasdermin-family genes are implicated in the regulation of apoptosis in epithelial cells, and are linked to cancer. Alternative splicing and the use of alternative promoters results in multiple transcript variants. Additional variants have been described, but they are candidates for nonsense-mediated mRNA decay (NMD) and are unlikely to be protein-coding.
Purification Method
Affinity purification
Gene ID
55876
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HT-29+NK-92, using Cleaved Gasdermin B Rabbit pAb (CAB23046) at 1:400 dilution. HT-29 and NK-92 cells were treated with IFNγ (50ng/ml) for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 120s.
