Anti-GSTT1 Antibody (CAB1287)

Antibody Name:	Anti-GSTT1 Antibody
Antibody SKU:	CAB1287
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human GSTT1 (NP_000844.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human GSTT1 (NP_000844.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGLE LYLD LLSQ PCRA VYIF AKKN DIPF ELRI VDLI KGQH LSDA FAQV NPLK KVPA LKDG DFTL TESV AILL YLTR KYKV PDYW YPQD LQAR ARVD EYLA WQHT TLRR SCLR ALWH KVMF PVFL GEPV SPQT LAAT LAEL DVTL QLLE DKFL QNKA FLTG PHIS LADL VAIT ELMH PVGA GCQV FEGR PKLA TWRQ RVEA AVGE DLFQ EAHE VILK AKDF PPAD PTIK QKLM PWVL AMIR
Gene ID:	2952
Uniprot:	P30711
Cellular Location:	Cytoplasm
Calculated MW:	13kDa/27kDa
Observed MW:	27kDa

Synonyms:

GSTT1

Background:

The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT1 and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	GSTT1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. Belongs to the GST superfamily. Theta family.
UniProt Protein Details:	Protein type:Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; Transferase; EC 2.5.1.18 Chromosomal Location of Human Ortholog: 22q11.23 Cellular Component: cytosol Molecular Function:glutathione peroxidase activity; glutathione transferase activity Biological Process: glutathione metabolic process
NCBI Summary:	The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT1 and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2015]
UniProt Code:	P30711
NCBI GenInfo Identifier:	21264427
NCBI Gene ID:	2952
NCBI Accession:	P30711.4
UniProt Secondary Accession:	P30711,O00226, Q5TZY2, Q6IC69, Q969K8, Q96IY3,
UniProt Related Accession:	P30711
Molecular Weight:	13,517 Da
NCBI Full Name:	Glutathione S-transferase theta-1
NCBI Synonym Full Names:	glutathione S-transferase theta 1
NCBI Official Symbol:	GSTT1
NCBI Protein Information:	glutathione S-transferase theta-1
UniProt Protein Name:	Glutathione S-transferase theta-1
UniProt Synonym Protein Names:	GST class-theta-1; Glutathione transferase T1-1
Protein Family:	Glutathione S-transferase
UniProt Gene Name:	GSTT1
UniProt Entry Name:	GSTT1_HUMAN

	Western blot analysis of extracts of various cell lines, using GSTT1 antibody (CAB1287) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded Mouse kidney using GSTT1 Rabbit pAb (CAB1287) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat kidney using GSTT1 Rabbit pAb (CAB1287) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human breast cancer using GSTT1 Rabbit pAb (CAB1287) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using GSTT1 Rabbit pAb (CAB1287) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-GSTT1 Antibody (CAB13996)	Human GSTt1 / Glutathione S Transferase Theta 1 ELISA Kit
GSTT1 Antibody (PACO09622)	Human GSTtheta1/GSTt1 (Glutathione S Transferase Theta 1) CLIA Kit (HUES00771)
	Human GSTtheta1/GSTt1 (Glutathione S Transferase Theta 1) ELISA Kit (HUES02381)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)