The Guinea Pig Insulin ELISA Kit offered by Assay Genie is a cutting-edge solution for precise measurement of insulin levels in guinea pig samples including serum, plasma, and tissue homogenates. This advanced kit is characterized by its exceptional sensitivity and selectivity, ensuring accurate and consistent results for various experimental needs. Insulin, a key hormone in regulating glucose metabolism, plays a vital role in maintaining blood sugar levels within the body. Fluctuations in insulin levels can have significant implications for metabolic disorders such as diabetes, making the Guinea Pig Insulin ELISA Kit an invaluable tool for researchers investigating these conditions and related therapeutics.
By utilizing this innovative ELISA kit, scientists can gain valuable insights into insulin dynamics in guinea pig models, paving the way for a deeper understanding of metabolic processes and potential therapeutic interventions. Assay Genie's Guinea Pig Insulin ELISA Kit is a reliable and efficient solution for studies focusing on insulin biology and its implications in metabolic health.
Product Name:
Guinea pig INS (Insulin) ELISA Kit
SKU:
AEFI00062
Reactivity:
Guinea pig
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
4.688 pg/mL
Range:
7.813-500 pg/mL (1ng=10uIU)
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-102%
83-101%
81-96%
EDTA Plasma (n = 5)
87-99%
82-100%
81-94%
Heparin Plasma (n = 5)
87-102%
90-100%
85-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-104
94
EDTA Plasma (n = 5)
86-95
91
Heparin Plasma (n = 5)
85-98
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
