Description
HAdV-41 E1B-55K Recombinant Protein (N-His)
HAdV-41 E1B-55K Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Lys98-Asp472, with an N-terminal His tag. Predicted molecular weight: 44.99 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. E1B 55 kDa protein (E1B-55K) is a ~52 kDa protein. Plays a major role to prevent cellular inhibition of viral genome replication. For research use only.
Assembles an SCF-like E3 ubiquitin ligase complex based on the cellular proteins ELOB, ELOC, CUL5 and RBX1, in cooperation with viral E4orf6. This viral RING-type ligase ubiquitinates cellular substrates and targets them to proteasomal degradation: TP53/p53, LIG4, MRE11-RAD50-NBS1 (MRN) complex, ITGA3, DAXX and BLM. E1B-55K probably acts as the substrate-specific adapter of the SCF-like E3 ubiquitin ligase complex. Degradation of host TP53/p53 activity is essential for preventing E1A-induced TP53 accumulation that would otherwise lead to cell apoptosis and growth arrest.
| Product Name: | HAdV-41 E1B-55K Recombinant Protein (N-His) |
| SKU: | GXRP07932 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | HAdV-41 |
| Synonyms: | E1B 55 kDa protein, E1B-55K, E1B protein, large T-antigen, E1B-495R |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Lys98-Asp472 |
| Calculated MW: | 44.99 kDa |
| UniProt: | P10546 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
