Anti-HAL Antibody (CAB13021)

Antibody Name:	Anti-HAL Antibody
Antibody SKU:	CAB13021
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HAL (NP_002099.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HAL (NP_002099.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPRY TVHV RGEW LAVP CQDA QLTV GWLG REAV RRYI KNKP DNGG FTSV DDAH FLVR RCKG LGLL DNED RLEV ALEN NEFV EVVI EGDA MSPD FIPS QPEG VYLY SKYR EPEK YIEL DGDR LTTE DLVN LGKG RYKI KLTP TAEK RVQK SREV IDSI IKEK TVVY GITT GFGK FART VIPI NKLQ ELQV NLVR SHSS GVGK
Gene ID:	3034
Uniprot:	P42357
Cellular Location:
Calculated MW:	49kDa/64kDa/72kDa
Observed MW:	73kDa

Synonyms:

HAL, HIS, HSTD

Background:

Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. Several transcript variants encoding different isoforms have been found for this gene.

NCBI Summary:	Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:	P42357
NCBI GenInfo Identifier:	4504333
NCBI Gene ID:	3034
NCBI Accession:	NP_002099.1
UniProt Related Accession:	P42357
Molecular Weight:	73kDa
NCBI Full Name:	histidine ammonia-lyase isoform 1
NCBI Synonym Full Names:	histidine ammonia-lyase
NCBI Official Symbol:	HAL
NCBI Official Synonym Symbols:	HIS; HSTD
NCBI Protein Information:	histidine ammonia-lyase
UniProt Protein Name:	Histidine ammonia-lyase
Protein Family:	Halocin
UniProt Gene Name:	HAL
UniProt Entry Name:	HUTH_HUMAN

	Western blot analysis of extracts of various cell lines, using HAL antibody (CAB13021) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using HAL antibody (CAB13021) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using HAL antibody (CAB13021) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human thyroid cancer using HAL antibody (CAB13021) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using HAL antibody (CAB13021) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)