Anti-HAT1 Antibody (CAB6188)

Antibody Name:	Anti-HAT1 Antibody
Antibody SKU:	CAB6188
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP ChIP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 245-419 of human HAT1 (NP_003633.1).

Application:	WB IHC IF IP ChIP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 ChIP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, SW480, Jurkat, 293T, HeLa, HepG2, U-251MG, Mouse spleen, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 245-419 of human HAT1 (NP_003633.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TPFQ GQGH GAQL LETV HRYY TEFP TVLD ITAE DPSK SYVK LRDF VLVK LCQD LPCF SREK LMQG FNED MAIE AQQK FKIN KQHA RRVY EILR LLVT DMSD AEQY RSYR LDIK RRLI SPYK KKQR DLAK MRKC LRPE ELTN QMNQ IEIS MQHE QLEE SFQE LVED YRRV IERL AQE
Gene ID:	8520
Uniprot:	O14929
Cellular Location:	Cytoplasm, Nucleus, Nucleus matrix, nucleoplasm
Calculated MW:	39kDa/49kDa
Observed MW:	50kDa

Synonyms:

HAT1, KAT1

Background:

The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.

UniProt Protein Function:	HAT1: Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage. Belongs to the HAT1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Acetyltransferase; EC 2.3.1.48 Chromosomal Location of Human Ortholog: 2q31.2-q33.1 Cellular Component: intracellular membrane-bound organelle; nuclear chromatin; nuclear chromosome, telomeric region; nucleoplasm; nucleus; protein complex Molecular Function:H4 histone acetyltransferase activity; histone acetyltransferase activity; histone binding; protein binding Biological Process: DNA packaging; DNA replication-dependent nucleosome assembly; DNA replication-independent nucleosome assembly; internal protein amino acid acetylation
NCBI Summary:	The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jun 2009]
UniProt Code:	O14929
NCBI GenInfo Identifier:	3334209
NCBI Gene ID:	8520
NCBI Accession:	O14929.1
UniProt Secondary Accession:	O14929,Q49A44, Q53QF0, Q53SU4, Q6P594, Q8WWB9,
UniProt Related Accession:	O14929
Molecular Weight:	39,786 Da
NCBI Full Name:	Histone acetyltransferase type B catalytic subunit
NCBI Synonym Full Names:	histone acetyltransferase 1
NCBI Official Symbol:	HAT1
NCBI Official Synonym Symbols:	KAT1
NCBI Protein Information:	histone acetyltransferase type B catalytic subunit
UniProt Protein Name:	Histone acetyltransferase type B catalytic subunit
UniProt Synonym Protein Names:	Histone acetyltransferase 1
Protein Family:	HAT1-interacting factor
UniProt Gene Name:	HAT1
UniProt Entry Name:	HAT1_HUMAN

Western blot analysis of extracts of various cell lines, using KAT1/KAT1/HAT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Antibodies	ELISA Kits
Anti-KAT1 / HAT1 Antibody (CAB4423)	HAT Colorimetric Cell-Based ELISA Kit
HAT1 Antibody (PACO05561)
HAT1 Antibody (PACO09674)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)