Description
HBcAg/HBV Capsid Recombinant Protein (C-His)
HBcAg/HBV Capsid Recombinant Protein (C-His) is a recombinant protein expressed in E. coli, spanning Met1-Val149, with a C-terminal His tag. Predicted molecular weight: 17.94 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Capsid protein (HBcAg) is a ~21 kDa protein. Self assembles to form an icosahedral capsid. For research use only.
Most capsids appear to be large particles with an icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsids are transported along microtubules to the nucleus. Phosphorylation of the capsid is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex via the importin (karyopherin-) alpha and beta. Capsids are imported in intact form through the nuclear pore into the nuclear basket, where it probably binds NUP153.
| Product Name: | HBcAg/HBV Capsid Recombinant Protein (C-His) |
| SKU: | GXRP03634 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | HBV |
| Synonyms: | Capsid protein, C, Core antigen, Core protein, HBcAg, p21.5 |
| Tag: | C-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Val149 |
| Calculated MW: | 17.94 kDa |
| UniProt: | P03146 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
