Western blot analysis of extracts of K-562 cells, using HBG2 antibody (CAB7683) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The HBG2 Polyclonal Antibody (CAB7683) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it reliable for use in various immunoassays such as Western blot and immunohistochemistry.HBG2, also known as fetal hemoglobin subunit gamma, plays a crucial role in the production of fetal hemoglobin during embryonic development. Dysregulation of HBG2 expression has been linked to various hematological disorders, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HBG2 Polyclonal Antibody
SKU:
CAB7683
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
MGHF TEED KATI TSLW GKVN VEDA GGET LGRL LVVY PWTQ RFFD SFGN LSSA SAIM GNPK VKAH GKKV LTSL GDAI KHLD DLKG TFAQ LSEL HCDK LHVD
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TNCY, HBG-T1, HBG2
Positive Sample:
K-562
Cellular Localization:
Blood Microparticle, Cytosol, Hemoglobin Complex.
Calculated MW:
16kDa
Observed MW:
13kDa
The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'- epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.
Purification Method
Affinity purification
Gene ID
3048
RRID
AB_2769745
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from K-562 cells, using HBG2 Rabbit pAb (CAB7683) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
