Anti-HDGF Antibody (CAB5347)

Antibody Name:	Anti-HDGF Antibody
Antibody SKU:	CAB5347
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HDGF (NP_004485.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	22Rv1, HeLa, A-549, 293T, U-251MG, MCF7, BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HDGF (NP_004485.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSRS NRQK EYKC GDLV FAKM KGYP HWPA RIDE MPEA AVKS TANK YQVF FFGT HETA FLGP KDLF PYEE SKEK FGKP NKRK GFSE GLWE IENN PTVK ASGY QSSQ KKSC VEEP EPEP EAAE GDGD KKGN AEGS SDEE GKLV IDEP AKEK NEKG ALKR RAGD LLED SPKR PKEA ENPE GEEK EAAT LEVE RPLP MEVE KNST PSEP GSGR GPPQ EEEE EEDE EEEA TKED AEAP GIRD HESL
Gene ID:	3068
Uniprot:	P51858
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	25kDa/26kDa/28kDa
Observed MW:	38kDa

Synonyms:

HDGF, HMG1L2

Background:

This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.

UniProt Protein Function:	Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.
NCBI Summary:	This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016]
UniProt Code:	P51858
NCBI GenInfo Identifier:	1708157
NCBI Gene ID:	3068
NCBI Accession:	P51858.1
UniProt Secondary Accession:	P51858,Q5SZ07, Q5SZ08, Q5SZ09, B3KU21, D3DVC9,
UniProt Related Accession:	P51858
Molecular Weight:	28,264 Da
NCBI Full Name:	Hepatoma-derived growth factor
NCBI Synonym Full Names:	hepatoma-derived growth factor
NCBI Official Symbol:	HDGF
NCBI Official Synonym Symbols:	HMG1L2
NCBI Protein Information:	hepatoma-derived growth factor
UniProt Protein Name:	Hepatoma-derived growth factor
UniProt Synonym Protein Names:	High mobility group protein 1-like 2; HMG-1L2
Protein Family:	Hepatoma-derived growth factor
UniProt Gene Name:	HDGF
UniProt Entry Name:	HDGF_HUMAN

	Western blot analysis of extracts of various cell lines, using HDGF antibody (CAB5347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat liver using HDGF Antibody (CAB5347) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using HDGF Antibody (CAB5347) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using HDGF Antibody (CAB5347) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using HDGF antibody (CAB5347) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-HDGF Antibody (CAB13654)	Human HDGF (Hepatoma Derived Growth Factor) ELISA Kit (HUFI07820)
HDGF Antibody (PACO09698)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)