The HFE Antibody (CAB1310) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the HFE protein, this antibody enables accurate detection and analysis in various cell types, making it an essential tool for studies in the fields of hematology, iron metabolism, and related disorders.HFE, also known as the hemochromatosis protein, is crucial for the regulation of iron absorption and distribution in the body.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
HFE Antibody
SKU:
CAB1310
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HH, HFE1, HLA-H, MVCD7, TFQTL2, HFE
Positive Sample:
Mouse liver, Mouse heart
Cellular Localization:
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
40kDa
Observed MW:
50kDa
The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene.
Purification Method
Affinity purification
Gene ID
3077
RRID
AB_2759949
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HFE Rabbit pAb (CAB1310) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
