Anti-HMGCR Antibody (CAB1633)

Antibody Name:	Anti-HMGCR Antibody
Antibody SKU:	CAB1633
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 629-888 of human HMGCR (NP_000850.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:1000 - 1:4000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse spleen, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 629-888 of human HMGCR (NP_000850.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ARLQ KLHT SIAG RNLY IRFQ SRSG DAMG MNMI SKGT EKAL SKLH EYFP EMQI LAVS GNYC TDKK PAAI NWIE GRGK SVVC EAVI PAKV VREV LKTT TEAM IEVN INKN LVGS AMAG SIGG YNAH AANI VTAI YIAC GQDA AQNV GSSN CITL MEAS GPTN EDLY ISCT MPSI EIGT VGGG TNLL PQQA CLQM LGVQ GACK DNPG ENAR QLAR IVCG TVMA GELS LMAA LAAG HLVK SHMI HNRS KINL QDLQ GACT KKTA
Gene ID:	3156
Uniprot:	P04035
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	92kDa/97kDa/99kDa
Observed MW:	97KDa

Synonyms:

HMGCR, LDLCQ3, HMGCR

Background:

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.
UniProt Protein Details:	Protein type:EC 1.1.1.34; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Endoplasmic reticulum; Membrane protein, integral; Motility/polarity/chemotaxis; Oxidoreductase; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 5q13.3-q14 Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane Molecular Function:protein binding; hydroxymethylglutaryl-CoA reductase (NADPH) activity; protein homodimerization activity; hydroxymethylglutaryl-CoA reductase activity; coenzyme binding Biological Process: negative regulation of MAP kinase activity; isoprenoid biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of skeletal muscle development; cellular lipid metabolic process; cholesterol biosynthetic process; positive regulation of stress-activated MAPK cascade; coenzyme A metabolic process; myoblast differentiation; ubiquinone metabolic process; response to ethanol; negative regulation of vasodilation; visual learning; protein tetramerization; response to nutrient; aging
NCBI Summary:	HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:	P04035
NCBI GenInfo Identifier:	123343
NCBI Gene ID:	3156
NCBI Accession:	P04035.1
UniProt Secondary Accession:	P04035,Q8N190, B7Z3Y9,
UniProt Related Accession:	P04035
Molecular Weight:	888
NCBI Full Name:	3-hydroxy-3-methylglutaryl-coenzyme A reductase
NCBI Synonym Full Names:	3-hydroxy-3-methylglutaryl-CoA reductase
NCBI Official Symbol:	HMGCR
NCBI Official Synonym Symbols:	LDLCQ3
NCBI Protein Information:	3-hydroxy-3-methylglutaryl-Coenzyme A reductase; HMG-CoA reductase; hydroxymethylglutaryl-CoA reductase; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH)
UniProt Protein Name:	3-hydroxy-3-methylglutaryl-coenzyme A reductase
UniProt Gene Name:	HMGCR
UniProt Entry Name:	HMDH_HUMAN

	Western blot analysis of extracts of Rat brain, using HMGCR Rabbit pAb (CAB1633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts of Mouse spleen, using HMGCR Rabbit pAb (CAB1633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human small intestine using HMGCR antibody (CAB1633) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human uterine cancer using HMGCR antibody (CAB1633) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of C6 cells using HMGCR Rabbit pAb (CAB1633) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-HMGCR Antibody (CAB13314)	Human HMGCR ELISA Kit
Anti-HMGCR Antibody (CAB14741)[KO Validated]	Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) ELISA Kit
Anti-HMGCR Antibody (CAB16875)
Anti-HMGCR Antibody (CAB16876)[KO Validated]
Anti-HMGCR Antibody (CAB19063)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)