Anti-HNMT Antibody (CAB2520)

Antibody Name:	Anti-HNMT Antibody
Antibody SKU:	CAB2520
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human HNMT (NP_008826.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	22RV1, NCI-H460, HT-29, HepG2, HeLa, Mouse liver, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human HNMT (NP_008826.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASS MRSL FSDH GKYV ESFR RFLN HSTE HQCM QEFM DKKL PGII GRIG DTKS EIKI LSIG GGAG EIDL QILS KVQA QYPG VCIN NEVV EPSA EQIA KYKE LVAK TSNL ENVK FAWH KETS SEYQ SRML EKKE LQKW DFIH MIQM LYYV KDIP ATLK FFHS LLGT NAKM LIIV VSGS SGWD KLWK KYGS RFPQ DDLC QYIT SDDL TQML DNLG LKYE CYDL LSTM DISD CFID GNEN GDLL WDFL TETC NFNA TAPP DLRA ELGK DLQE PEFS AKKE GKVL FNNT LSFI VIEA
Gene ID:	3176
Uniprot:	P50135
Cellular Location:	Cytoplasm
Calculated MW:	6kDa/14kDa/33kDa
Observed MW:	33kDa

Synonyms:

HNMT, HMT, HNMT-S1, HNMT-S2, MRT51

Background:

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.

UniProt Protein Function:	HNMT: Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine. Belongs to the methyltransferase superfamily. HNMT family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - histidine; EC 2.1.1.8; Methyltransferase Chromosomal Location of Human Ortholog: 2q22.1 Cellular Component: cytoplasm; cytosol; neuron projection; nucleoplasm Molecular Function:histamine N-methyltransferase activity Biological Process: brain development; histidine catabolic process; methylation; respiratory gaseous exchange Disease: Asthma, Susceptibility To
NCBI Summary:	In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P50135
NCBI GenInfo Identifier:	1708272
NCBI Gene ID:	3176
NCBI Accession:	P50135.1
UniProt Secondary Accession:	P50135,Q546Z6, Q7Z7I2, Q8IU56, Q8WW98, Q9BRW6, B2R9J3
UniProt Related Accession:	P50135
Molecular Weight:	6,046 Da
NCBI Full Name:	Histamine N-methyltransferase
NCBI Synonym Full Names:	histamine N-methyltransferase
NCBI Official Symbol:	HNMT
NCBI Official Synonym Symbols:	HMT; MRT51; HNMT-S1; HNMT-S2
NCBI Protein Information:	histamine N-methyltransferase
UniProt Protein Name:	Histamine N-methyltransferase
Protein Family:	Histamine N-methyltransferase
UniProt Gene Name:	HNMT
UniProt Entry Name:	HNMT_HUMAN

	Western blot analysis of extracts of various cell lines, using HNMT antibody (CAB2520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using HNMT antibody (CAB2520). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 1ug HNMT antibody (CAB2520). Western blot was performed from the immunoprecipitate using HNMT antibody (CAB2520) at a dilition of 1:1000.____Detection: ECL Enhanced Kit (RM00021)._Exposure time: 10s.

Antibodies	ELISA Kits
Anti-HNMT Antibody (CAB13499)	Human HNMT(Histamine N-methyltransferase) ELISA Kit
HNMT Antibody (PACO09757)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)