Western blot analysis of extracts of various cell lines, using hnRNP E2/PCBP2 antibody (CAB21119) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The hnRNP E2/PCBP2 Monoclonal Antibody (CAB21119) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in mice, specifically targets the HNRNP E2/PCBP2 protein and is highly reactive with human samples. It has been validated for use in various applications including Western blot and immunohistochemistry, allowing for accurate detection and analysis of HNRNP E2/PCBP2 in different cell types.HNRNP E2/PCBP2, also known as heterogeneous nuclear ribonucleoprotein E2 or poly(rC)-binding protein 2, is a multifunctional protein that plays a key role in processes such as pre-mRNA splicing, mRNA stability, and translation regulation.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
hnRNP E2/PCBP2 Monoclonal Antibody
SKU:
CAB21119
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2994
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HNRPE2, HNRNPE2, hnRNP-E2, hnRNP E2/PCBP2
Positive Sample:
HeLa, NIH/3T3, Mouse testis, C6
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
39kDa
Observed MW:
35-45kDa
The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5094
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using hnRNP E2/PCBP2 Rabbit mAb (CAB21119) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug hnRNP E2/PCBP2 antibody (CAB21119). Western blot was performed from the immunoprecipitate using hnRNP E2/PCBP2 antibody (CAB21119) at a dilution of 1:1000.
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