Anti-HNRNPD Antibody (CAB1828)

Antibody Name:	Anti-HNRNPD Antibody
Antibody SKU:	CAB1828
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of human HNRNPD (NP_002129.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, Jurkat, 293T, HepG2, HeLa, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of human HNRNPD (NP_002129.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSEE QFGG DGAA AAAT AAVG GSAG EQEG AMVA ATQG AAAA AGSG AGTG GGTA SGGT EGGS AESE GAKI DASK NEED EGHS NSSP RHSE AATA QREE WKMF IGGL SWDT TKKD LKDY FSKF GEVV DCTL KLDP ITGR SRGF GFVL FKES ESVD KVMD QKEH KLNG KVID PKRA KAMK TKEP VKKI FVGG LSPD TPEE KIRE YFGG FGEV ESIE LPMD NKTN KRRG FCFI TFKE EEPV KKIM EKKY HNVG LSKC EIKV AMSK EQYQ QQQQ WGSR GGFA GRAR GRGG DQQS GYGK VSRR GGHQ NSYK PY
Gene ID:	3184
Uniprot:	Q14103
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	30kDa/32kDa/36kDa/38kDa
Observed MW:	43kDa

Synonyms:

HNRNPD, AUF1, AUF1A, HNRPD, P37, hnRNPD0

Background:

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.

UniProt Protein Function:	Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. May play a role in the regulation of the rhythmic expression of circadian clock core genes. Directly binds to the 3'UTR of CRY1 mRNA and induces CRY1 rhythmic translation. May also be involved in the regulation of PER2 translation.
NCBI Summary:	This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008]
UniProt Code:	Q14103
NCBI GenInfo Identifier:	13124489
NCBI Gene ID:	3184
NCBI Accession:	Q14103.1
UniProt Secondary Accession:	Q14103,P07029, Q01858, Q14100, Q14101, Q14102, Q4W5A1 Q9UCE8, Q9UCE9, A8K9J2,
UniProt Related Accession:	Q14103
Molecular Weight:	30,672 Da
NCBI Full Name:	Heterogeneous nuclear ribonucleoprotein D0
NCBI Synonym Full Names:	heterogeneous nuclear ribonucleoprotein D
NCBI Official Symbol:	HNRNPD
NCBI Official Synonym Symbols:	P37; AUF1; AUF1A; HNRPD; hnRNPD0
NCBI Protein Information:	heterogeneous nuclear ribonucleoprotein D0
UniProt Protein Name:	Heterogeneous nuclear ribonucleoprotein D0
UniProt Synonym Protein Names:	AU-rich element RNA-binding protein 1
Protein Family:	Heterogeneous nuclear ribonucleoprotein
UniProt Gene Name:	HNRNPD
UniProt Entry Name:	HNRPD_HUMAN

	Western blot analysis of extracts of various cell lines, using HNRNPD antibody (CAB1828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using HNRNPD Antibody (CAB1828) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using HNRNPD Antibody (CAB1828) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse pancreas using HNRNPD Antibody (CAB1828) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using HNRNPD antibody (CAB1828).

Antibodies	ELISA Kits
Anti-HNRNPD Antibody (CAB15679)	hnRPD (Phospho-Ser83) Colorimetric Cell-Based ELISA Kit
HNRNPD Antibody (PACO00924)
Phospho-HNRNPD (S83) Antibody (PACO04058)
HNRNPD Antibody (PACO09762)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)